Characterization of circulating breast cancer cells with tumorigenic and metastatic capacity

ABSTRACT: Functional studies giving insight into the biology of circulating tumor cells (CTCs) remain scarce due to the low frequency of CTCs and lack of appropriate models. Here, we describe the characterization of a novel CTC-derived breast cancer cell line, designated CTC-ITB-01, established from a patient with metastatic estrogen receptor-positive (ER+) breast cancer, resistant to endocrine therapy. CTC-ITB-01 remained ER+ in culture, and copy number alteration (CNA) profiling showed high concordance between CTC-ITB-01 and CTCs originally present in the cancer patient at the time point of blood draw. RNA sequencing data indicate that CTC-ITB-01 has a predominantly epithelial expression signature. Primary tumor and metastasis formation in an intra-ductal PDX mouse model mirrored the clinical prog

SUBMITTER: Mrs. Claudia Koch 

PROVIDER: S-SCDT-EMM-2019-11908 | biostudies-other |

REPOSITORIES: biostudies-other