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A physical sciences network characterization of non-tumorigenic and metastatic cells.


ABSTRACT: To investigate the transition from non-cancerous to metastatic from a physical sciences perspective, the Physical Sciences-Oncology Centers (PS-OC) Network performed molecular and biophysical comparative studies of the non-tumorigenic MCF-10A and metastatic MDA-MB-231 breast epithelial cell lines, commonly used as models of cancer metastasis. Experiments were performed in 20 laboratories from 12 PS-OCs. Each laboratory was supplied with identical aliquots and common reagents and culture protocols. Analyses of these measurements revealed dramatic differences in their mechanics, migration, adhesion, oxygen response, and proteomic profiles. Model-based multi-omics approaches identified key differences between these cells' regulatory networks involved in morphology and survival. These results provide a multifaceted description of cellular parameters of two widely used cell lines and demonstrate the value of the PS-OC Network approach for integration of diverse experimental observations to elucidate the phenotypes associated with cancer metastasis.

SUBMITTER: Physical Sciences - Oncology Centers Network 

PROVIDER: S-EPMC3636513 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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A physical sciences network characterization of non-tumorigenic and metastatic cells.

Agus David B DB   Alexander Jenolyn F JF   Arap Wadih W   Ashili Shashanka S   Aslan Joseph E JE   Austin Robert H RH   Backman Vadim V   Bethel Kelly J KJ   Bonneau Richard R   Chen Wei-Chiang WC   Chen-Tanyolac Chira C   Choi Nathan C NC   Curley Steven A SA   Dallas Matthew M   Damania Dhwanil D   Davies Paul C W PC   Decuzzi Paolo P   Dickinson Laura L   Estevez-Salmeron Luis L   Estrella Veronica V   Ferrari Mauro M   Fischbach Claudia C   Foo Jasmine J   Fraley Stephanie I SI   Frantz Christian C   Fuhrmann Alexander A   Gascard Philippe P   Gatenby Robert A RA   Geng Yue Y   Gerecht Sharon S   Gillies Robert J RJ   Godin Biana B   Grady William M WM   Greenfield Alex A   Hemphill Courtney C   Hempstead Barbara L BL   Hielscher Abigail A   Hillis W Daniel WD   Holland Eric C EC   Ibrahim-Hashim Arig A   Jacks Tyler T   Johnson Roger H RH   Joo Ahyoung A   Katz Jonathan E JE   Kelbauskas Laimonas L   Kesselman Carl C   King Michael R MR   Konstantopoulos Konstantinos K   Kraning-Rush Casey M CM   Kuhn Peter P   Kung Kevin K   Kung Kevin K   Kwee Brian B   Lakins Johnathon N JN   Lambert Guillaume G   Liao David D   Licht Jonathan D JD   Liphardt Jan T JT   Liu Liyu L   Lloyd Mark C MC   Lyubimova Anna A   Mallick Parag P   Marko John J   McCarty Owen J T OJ   Meldrum Deirdre R DR   Michor Franziska F   Mumenthaler Shannon M SM   Nandakumar Vivek V   O'Halloran Thomas V TV   Oh Steve S   Pasqualini Renata R   Paszek Matthew J MJ   Philips Kevin G KG   Poultney Christopher S CS   Rana Kuldeepsinh K   Reinhart-King Cynthia A CA   Ros Robert R   Semenza Gregg L GL   Senechal Patti P   Shuler Michael L ML   Srinivasan Srimeenakshi S   Staunton Jack R JR   Stypula Yolanda Y   Subramanian Hariharan H   Tlsty Thea D TD   Tormoen Garth W GW   Tseng Yiider Y   van Oudenaarden Alexander A   Verbridge Scott S SS   Wan Jenny C JC   Weaver Valerie M VM   Widom Jonathan J   Will Christine C   Wirtz Denis D   Wojtkowiak Jonathan J   Wu Pei-Hsun PH  

Scientific reports 20130101


To investigate the transition from non-cancerous to metastatic from a physical sciences perspective, the Physical Sciences-Oncology Centers (PS-OC) Network performed molecular and biophysical comparative studies of the non-tumorigenic MCF-10A and metastatic MDA-MB-231 breast epithelial cell lines, commonly used as models of cancer metastasis. Experiments were performed in 20 laboratories from 12 PS-OCs. Each laboratory was supplied with identical aliquots and common reagents and culture protocol  ...[more]

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