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Location and Function of Cytosine-5 Methylation in RNA

ABSTRACT:

Mutations in the cytosine-5 RNA methyltransferase NSun2 can cause neurodevelopmental disorders and symptoms commonly found in patients with Dubowitz-like syndrome. Some tRNAs are known to be methylated NSun2, however the occurrence of cytosine-5 methylation (m⁵C) in other RNA biotypes is still under debate. Location in RNA and function of m⁵C has not been studied yet. This study is aimed at identifying new m⁵C methylated RNA biotypes, as well as the location at specific structures or sequences and the ultimate biological function. The impact of the loss of NSun2-mediated methylation is also determined by comparing gene expression data with the global cytosine-5 RNA methylome in Dubowitz-like syndrome patients. We also use ribosomal profiling to assess the impact of the loss and rescue of Nsun2-mediated cytosine-5 RNA methylation on translation rates and ribosome occupancy.

PROVIDER: phs000645 | dbGaP |

SECONDARY ACCESSION(S): PRJNA209565PRJNA209566

REPOSITORIES: dbGaP

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			Action	DRS
	dbGaPEx2.1.5.xsd	Other
	datadict_v2.xsl	Other
	phs000645.v5.pht003740.v2.Intellectual_Disabilities_Subject.data_dict.xml	Xml
	phs000645.v5.pht003740.v2.p1.Intellectual_Disabilities_Subject.var_report.xml	Xml
	phs000645.v5.pht003741.v5.Intellectual_Disabilities_Sample.data_dict.xml	Xml

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