Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RAS Mutation Testing in the Circulating Blood of Patients With Metastatic Colorectal Cancer

ABSTRACT: This study will evaluate the concordance of RAS mutation detection between the results obtained from circulating tumor DNA and those obtained with the "standard" method (testing from tumor tissue).

DISEASE(S): Colorectal Cancer,Colorectal Neoplasms

PROVIDER: 2194501 | ecrin-mdr-crc |

REPOSITORIES: ECRIN MDR

ACCESS DATA

Json Xml

Similar Datasets

Use of lymph-circulating extracellular vesicles as surrogate markers of melanoma progression and BRAFV600E mutation

Project description:Liquid biopsies are becoming imperative on early patient diagnosis, prognosis and evaluation of residual disease. The use of circulating extracellular vesicles (EVs) as surrogate markers of tumor progression could be a powerful tool in the clinical setting. EVs in plasma have emerged as a non-invasive option to detect metastatic outcome, however sensitivity is low. Here we have characterized the lymph obtained after postoperative lymphadenectomy as a novel biological fluid enriched in EVs. Our proteomic profiling and BRAFV600E/K status determination demonstrate for the first time that EVs from the lymph of melanoma patients are enriched in melanoma-associated proteins and are useful for BRAF mutations detection. Melanoma oncogenic pathways, immunomodulation and platelet activating proteins are enriched in lymph-derived exosomes from patients with distal lymph node spread compared to local/early spreading. Furthermore, patients positive for BRAFV600E mutation on lymph-circulating vesicles had a shorter time of relapse. These data encourage the analysis of lymph-circulating EVs for detection of residual disease and recurrence.

2019-03-13 | PXD012042 | Pride

Evaluation of RAS gene status in circulating tumor DNA for colorectal cancer patients with RASg gene mutation

Project description:Primary outcome(s): the allele frequency of RAS gene mutation in circulating tumor DNA

| 2653459 | ecrin-mdr-crc

Use of lymph-circulating extracellular vesicles as surrogates of melanoma progression markers and BRAFV600E mutation.

2021-02-26 | PXD009505 | Pride

Circulating tumor DNA sequencing provides comprehensive mutation profiling for pediatric central nervous system tumors

Project description:Circulating tumor DNA sequencing provides comprehensive mutation profiling for pediatric central nervous system tumors

| PRJNA865269 | ENA

Circulating tumor DNA sequencing provides comprehensive mutation profiling for pediatric central nervous system tumors

Project description:Circulating tumor DNA sequencing provides comprehensive mutation profiling for pediatric central nervous system tumors

| PRJNA869170 | ENA

Circulating tumor DNA sequencing provides comprehensive mutation profiling for pediatric central nervous system tumors

Project description:Circulating tumor DNA sequencing provides comprehensive mutation profiling for pediatric central nervous system tumors

| PRJNA869171 | ENA

Circulating RNAs profile in non-small cell lung cancer patients

Project description:Circulating RNAs are a less invasive and easy accessed source of samples for biomedical research and clinical applications. However, circulating mRNA is mostly fragmented and less abundant. High throughput RNA sequencing (RSEQ) and DASL assay have been both applied to profile such fragmented RNA samples. In this study, we compared the ability of transcriptomic profiling of the two platforms. Circulating RNAs from three non-small cell lung cancer patients and three age-matched healthy controls were analyzed by RSEQ and DASL assay. The concordance of each gene analyzed by the two platforms were measured with Pearson's correlation coefficient. And gene expression level determined by both platforms were confirmed by RT-PCR. The two platforms showed modest to moderate correlation. Genes with higher expression level represented higher cross-platform concordance. Compare the results of RT-PCR and the two platforms, RSEQ was much higher correlated with RT-PCR. This data suggested that RSEQ could be more suitable for circulating RNA profiling. In conclusion, we have demonstrated that genes with higher expression levels showed cross-platform concordance. And, the RSEQ could be more suitable tool for profiling circulating RNAs.

2015-10-06 | GSE69732 | GEO

Prospective Molecular Profiling of Circulating Tumor Cells from Patients with Melanoma Receiving Combinatorial Immunotherapy

Project description:Transcriptomic profiling of circulating tumor cells obtained from the blood of three melanoma patients on immunotherapy.

2018-06-26 | GSE100565 | GEO

Clinical validation study of RAKSET-B, a multiple detection kit for BRAF and RAS gene mutations in colorectal cancer

Project description:Primary outcome(s): BRAF gene mutation* - Overall concordance rate, positive concordance rate, and negative concordance rate of BRAF gene mutations between standard genetic testing such as sanger sequencing and RASKET-B kit RAS gene mutation** - Overall concordance rate, positive concordance rate, and negative concordance rate of RAS gene mutations between MEBGEN RASKET KIT (an established in vitro diagnostic (IVD) kit for detecting RAS mutations) and RASKET-B kit *BRAF gene mutation codon 600 (p.V600E) **RAS gene mutation codon 12, 13, 59, 61, 117, 146

| 2637999 | ecrin-mdr-crc

Recurrent activating mutation in PRKACA in cortisol-producing adrenal tumors

Project description:We performed mRNA-seq of a PRKACA-mutant adrenal tumor and demonstrated that the mutation is expressed at the mRNA level. Total RNA obtained from a cortisol-producing adrenal tumor with a PRKACA p.Leu206Arg mutation.

2014-04-15 | E-GEOD-56016 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data