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Dose combined spinal-general anesthesia improve postoperative quality of recovery in patients undergoing laparoscopic colorectal surgery?

ABSTRACT: Interventions: combined anesthesia group:Patient received intrathecal injection of 250 µg morphine with 15 mg ropivacaine combined general anesthesia;control group:Patient received simulate spinal anesthesia Primary outcome(s): Postoperative quality of recovery Study Design: Parallel

DISEASE(S): Colorectal Cancer

PROVIDER: 2741142 | ecrin-mdr-crc |

REPOSITORIES: ECRIN MDR

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Withdraw due to duplicate registration. Dose intrathecal morphine improve postoperative analgesia in patients undergoing laparoscopic colorectal surgery?

Project description:Interventions: 0.5 mg morphine group:Patient received intrathecal 0.5 mg morphine with 15 mg ropivacaine before general anesthesia;0.25 mg morphine group:Patient received intrathecal 0.25 mg morphine with 15 mg ropivacaine before general anesthesia;Ropivacaine group:Patient received intrathecal 15 mg ropivacaine before general anesthesia Primary outcome(s): Area under the curve of activity visual simulation pain score;Postoperative cumulative consumption of opioid analgesics Study Design: Parallel

| 2760431 | ecrin-mdr-crc

Inhibition of spinal cord Hsp90 enhances morphine anti-nociception by activating an ERK/RSK pathway

Project description:Morphine and other opioids continue to be commonly utilized as clinical analgesics, despite their numerous adverse side effects, including respiratory depression, addiction, and tolerance. The modulation of µ-opioid receptor (MOR) signaling and subsequent behavioral output is one viable approach to improve opioid therapy. Heat shock protein 90 (Hsp90) is a molecular chaperone protein that has recently been implicated in downstream MOR signaling within the brain in mice. Here we identify a context-dependent impact on MOR signaling in which the inhibition of Hsp90 within the spinal cord promotes morphine induced anti-nociception. We show that intrathecal and not systemic administration of the Hsp90 inhibitors 17-AAG or KU-32 amplifies morphine-induced anti-nociception in both thermal and mechanical pain models. Further, we demonstrate that the inhibition of Hsp90 allows for ERK phosphorylation after opioid treatment. ERK activation was localized within the dorsal horns of the spinal cord, which are heavily populated with primary afferents responsible for nociception. The behavioral effects observed with Hsp90 inhibitors were abolished upon intrathecal U0126 (ERK inhibitor) and cycloheximide (translation inhibitor) treatment, suggesting that downstream ERK phosphorylation and rapid protein translation are responsible for the observed amplification of anti-nociception. Quantitative proteomic analysis identified upregulated RSK with spinal cord Hsp90 inhibition, which we further found was necessary for the observed enhancement of anti-nociception. Taken together, we have uncovered a novel downstream MOR ERK/RSK cascade, localized to the spinal cord and repressed by Hsp90, whose modulation may allow for enhanced efficacy and decreased side effects during opioid therapy.

2020-04-24 | PXD015060 | Pride

Transcriptome analysis of the hippocampus in late postnatal mice after repeated ketamine anesthesia

Project description:Numerous recent studies showed that early anesthesia during neurodevelopment may induce changes in specific behaviors. We further studied the effects of early anesthetic exposures regarding addiction behavior later in life. Postnatal day (PND) 16 C57BL/6 mice received ketamine anesthesia for 5 consecutive days. Addiction behavior was evaluated 1 week after anesthesia treatment. Neurological changes after repeated anesthesia were evaluated using high-performance liquid chromatography, RNA sequencing, electrophysiology studies. Repeated ketamine anesthesia during the critical neurodevelopment period increased the expression of genes involved with action potential, and induced excitatory/inhibitory (E/I) imbalance in hippocampal CA1 pyramidal neurons of male mice. Such neurological changes were associated with increases in drug-induced contextual addiction memory.

2022-07-14 | GSE175894 | GEO

Effects of lidocaine on the expression of rat spinal cord

Project description:This study aimed to explore the possible mechanism of lidocaine-induced neurotoxicity. A total of 40 rats were randomly assigned to 4 groups (n = 10 per group): control group; sham group, received only intrathecal catheter insertion; saline group, received an intrathecal injection of saline; and lidocaine group, received an intrathecal injection of 10% lidocaine. Neurobehavioral tests were performed on rats in each group before injection and 1, 2, 3, and 4 days after the intrathecal injection. On the fourth day, after completing neurobehavioral tests, the spinal cords of all rats from L2 to L6 were removed and immediately stored in liquid nitrogen. In the control and lidocaine groups, parts of the spinal cord tissues of three rats were selected for gene microarray analysis.Based on the results of the gene chip analysis,Five rats in each group underwent real-time quantitative polymerase chain reaction (PCR) and five Western blot analysis to verify Differentially expressed genes at the mRNA transcription and protein levels. The aim of gene chip analysis is to screen potential genes involved in lidocaine-induced neurotoxicity.

2019-09-05 | GSE136833 | GEO

Effects of different doses of intrathecal morphine on quality of postoperative recovery in patients undergoing laparoscopic colorectal surgery

Project description:Interventions: 0.2mg morphine group:Intrathecal 0.2mg morphine + ropivacaine15mg;0.1mg morphine group:Intrathecal 0.1mg morphine + ropivacaine15mg;0.05mg morphine group:Intrathecal 0.05mg morphine + ropivacaine15mg;control group:Intrathecal ropivacaine15mg Primary outcome(s): Quality of Recovery-15 Study Design: Parallel

| 81681 | ecrin-mdr-crc

Effects of dexmedetomidine combined with epidural anesthesia on intestinal function and postoperative outcomes of patients undergoing laparoscopic colorectal cancer surgery

Project description:Interventions: Control group (CON group):Intravenous and inhalation combined anesthesia, equal amount of normal saline; Sufentanil PCIA.;Dexmedetomidine group (DEX group):Dexmedetomidine group (DEX group): intravenous anesthesia was assisted by intravenous of DEX with the loading dose of 0.5~1.0 microgram/kg completed within 10 minutes and maintenance infusion of 0.5~1.0 microgram /kg/h until the end of the operation. Sufentanil + DEX to implement PCIA.; Epidural group (EP group):Intravenous Inhalation Combined with epidural anesthesia, ropivacaine + sufentanil PCEA;DEX + EP group:Intravenous anesthesia assisted by intravenous DEX combined with epidural anesthesia, ropivacaine + DEX PCEA. Primary outcome(s): Peristalsis time after operation;Oral feeding time after operation Study Design: Parallel

| 47743 | ecrin-mdr-crc

Gene expression profiles in liver of Sprague-Dawley rat using two types of anesthesia.

Project description:To reveal influence of anesthesia in toxicogenomics study, gene expression analysis of liver of Sprague-Dawley rat after isoflurane anesthesia or CO2/O2 anesthesia was performed. The liver samples were excised from each rat after isoflurane anesthesia or CO2/O2 anesthesia. The gene expression profiles in liver were measureed by Agilent microarray.

2015-07-24 | GSE71277 | GEO

Expression data from sevoflurane anesthesia treated rat

Project description:Long noncoding RNAs (lncRNAs) play important roles in brain function modulation and neurodegenerative diseases. However, whether lncRNA regulations are involved in the mechanisms of perioperative neurocognitive disorders (PNCD), especially in anesthesia related brain dysfunction, remains unknown. We explored the expression and regulation pattern profiles of lncRNAs in the hippocampus of aged rats after sevoflurane anesthesia with microarrays.

2020-12-29 | GSE139220 | GEO

CircRNA-miRNA in anesthesia-induced permanent nerve injury during caesarean delivery

Project description:Permanent nerve damage caused by anesthesia is a rare but critical issue, and the mechanism of it remains unclear. Large-scale analysis of changes in noncoding RNA such as circRNA and microRNA in anesthesia-related nerve damage may provide insight into the mechanism of anesthesia-induced nerve injury.

2022-08-02 | GSE135297 | GEO

Single-cell RNA sequencing of microglia under awake and anesthesia-state

Project description:To probe potential differences between awake and anesthesia microglia, we performed the single-cell RNA sequencing (scRNA-seq) . Microglia were isolated by FACS as CD11b high CD45 low cells from awake and anesthesia mouse brains.

2023-08-07 | GSE217202 | GEO

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