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Withdraw due to duplicate registration. Dose intrathecal morphine improve postoperative analgesia in patients undergoing laparoscopic colorectal surgery?

ABSTRACT: Interventions: 0.5 mg morphine group:Patient received intrathecal 0.5 mg morphine with 15 mg ropivacaine before general anesthesia;0.25 mg morphine group:Patient received intrathecal 0.25 mg morphine with 15 mg ropivacaine before general anesthesia;Ropivacaine group:Patient received intrathecal 15 mg ropivacaine before general anesthesia Primary outcome(s): Area under the curve of activity visual simulation pain score;Postoperative cumulative consumption of opioid analgesics Study Design: Parallel

DISEASE(S): Colorectal Cancer

PROVIDER: 2760431 | ecrin-mdr-crc |

REPOSITORIES: ECRIN MDR

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Project description:Histone acetylation and other modifications of the chromatin are important regulators of gene expression and, consequently, may contribute to drug-induced behaviors and neuroplasticity. Previous studies have shown that a reduction on histone deacetylase (HDAC) activity results on the enhancement of some psychostimulant-induced behaviors. In the present study, we extend those seminal findings by showing that the administration of the HDAC inhibitor sodium butyrate enhances morphine-induced locomotor sensitization and conditioned place preference. In contrast, this compound has no effects on the development of morphine tolerance and dependence. Similar effects were observed for cocaine and ethanol-induced behaviors. These behavioral changes were accompanied by a selective boosting of a component of the transcriptional program activated by chronic morphine administration that included circadian clock genes and other genes relevant in addictive behavior. Our results support an specific role for histone acetylation and the epigenetic modulation of transcription at a reduced number of biologically relevant loci on non-homeostatic, long lasting, drug-induced behavioral plasticity. To further investigate the molecular bases of sodium butyrate action on long-lasting behavioral responses to morphine, we screened for potential substrates of their interaction by performing a genome-wide comparison of the striatal transcriptome after chronic administration of morphine in the absence or presence of sodium butyrate. Striatal tissue was dissected from two months-old Swiss-Albino male mice subjected to behavioral sensitization. Behavioural sensitization: Locomotor sensitization induced by morphine was evaluated using a protocol divided into two phases: induction and challenge. The induction phase involved six trials on alternate days, one trial per day. On each one of these trials, mice received and injection of saline or sodium butyrate (300 mg/kg) immediatedly followed by a second injection of morphine (20 mg/kg). The challenge phases consisted of a single trial conducted 7 days after the last test of the induction phase. In this case, all animals received a single injection of morphine (20 mg/kg). Striatal RNA was extracted 1h after the morphine challenge in groups of four animals that received either saline (N=6) or morphine (N=3), or the sodium butyrate-morphine (N=3) co-treatment during the sensitization induction phase.

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Withdraw due to duplicate registration. Dose intrathecal morphine improve postoperative analgesia in patients undergoing laparoscopic colorectal surgery?

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