ABSTRACT: Background and study aims
Colorectal cancer is a type of cancer that starts in the colon or rectum, often developing from growths called polyps.
The LAMP technique, or Loop-Mediated Isothermal Amplification, is a molecular biology method that allows scientists to rapidly and accurately amplify specific DNA sequences under constant temperature conditions. This technique has applications in various fields, including medical diagnostics, research, and disease detection. In the present study, our focus is on improving the LAMP technique by introducing a new method to analyze various samples. We plan to use colors and a substance called phenol red to make this possible, and we'll refer to it as Quantitative LAMP (QLAMP-phenol red).
The QLAMP-phenol red method aims to provide a precise way of measuring small things in biology, particularly beneficial for places with limited resources. It's designed to be straightforward, fast, accurate, reliable, and cost-effective. Additionally, the visual interpretation of color changes, observable with the naked eye, adds to its practicality.
In our research, we aim to identify a gene called fadA that could serve as a marker for detecting a specific bacteria called Fusobacterium nucleatum, associated with colorectal cancer. By designing specialized tools (akin to magnifying glasses) using the LAMP technique, we hope to enable early cancer detection in patients. Furthermore, we intend to enhance the LAMP technique's capabilities by integrating phenol red to ensure more precise measurements.
Who can participate?
Patients aged 18 - 80 years with colorectal cancer.
What does the study involve?
An administration of a Probiotic for 4 weeks. Stool sampling at baseline and 1, 2, 3, 4 weeks.
