Project description:Four ceftazidime-resistant isolates of a Klebsiella pneumoniae strain were collected from intensive care unit patients in Nijmegen, The Netherlands. These isolates had TEM-29 and SHV-14 beta-lactamases. SHV-14 is a novel variant, with two substitutions compared with the sequence of SHV-1: Ile8Phe and Arg43Ser. Its gene also had a silent C-->T mutation at nucleotide 481. The SHV-14 enzyme had slightly higher V(max) rates than SHV-1 for oxyimino-aminothiazolyl cephalosporins, but this activity was insufficient for the enzyme to count as an extended-spectrum beta-lactamase.

| S-EPMC90280 | biostudies-literature

Department of Clinical Genetics, Leiden University Medical Center, The Netherlands

Project description:Organisation EGAO00000000860

| EGAO00000000860 | EGA

Department of Human Genetics, Leiden University Medical Center, The Netherlands

Project description:Organisation EGAO00000000861

| EGAO00000000861 | EGA

The Netherlands Cancer Institute

Project description:Organisation EGAO00000001191

| EGAO00000001191 | EGA