Genomics

Dataset Information

0

Understanding human fetal pancreas development using subpopulation sorting and RNA sequencing


ABSTRACT: To decipher the populations of cells present in the human fetal pancreas and their lineage relationships, we developed strategies to isolate pancreatic progenitors, endocrine progenitors and endocrine cells. Transcriptome analysis of the individual populationsrevealed a large degree of conservation among vertebrates in the drivers of gene expression changes occurring at different steps of differentiation, althoughnotably, sometimes, different members of the same gene family are expressed. The transcriptome analysis establishes a resource to identify novel genes and pathways involved in human pancreas development. Single cell profiling further captured intermediate stages ofdifferentiation and enabled us to decipher the sequence of transcriptional events occurring during human endocrine differentiation. Furthermore, we evaluate how well individual pancreatic cells derived in vitro from human pluripotent stem cells mirror the natural processoccurring in human fetuses. This comparison uncovers a few differences at the progenitor steps, a convergence at the steps of endocrine induction and the current inability to fully resolve endocrine cell subtypes in vitro.

PROVIDER: EGAS00001003127 | EGA |

REPOSITORIES: EGA

Similar Datasets

2021-06-30 | GSE179119 | GEO
2021-06-30 | GSE179118 | GEO
2014-07-28 | E-GEOD-56617 | biostudies-arrayexpress
2014-07-28 | GSE56617 | GEO
2021-10-27 | GSE167596 | GEO
2021-10-27 | GSE167602 | GEO
2006-06-30 | E-CBIL-14 | biostudies-arrayexpress
2017-06-21 | MSV000081178 | MassIVE
2012-08-01 | E-GEOD-34060 | biostudies-arrayexpress
2020-06-02 | GSE144953 | GEO