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The transcriptional repressor REST is a major suppressor of endocrine differentiation during pancreas organogenesis [RNA-seq]

ABSTRACT: Purpose: Decipher the role of REST in pancreas organogenesis and endocrine differentiation (REST RNAseq-samples E18.5 pancreas)

ORGANISM(S): Mus musculus

PROVIDER: GSE179119 | GEO | 2021/06/30

REPOSITORIES: GEO

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The transcriptional repressor REST is a major suppressor of endocrine differentiation during pancreas organogenesis [ChIP-seq]

Project description:Purpose: Decipher the role of REST in pancreas organogenesis and endocrine differentiation (REST ChIPseq-samples E13.5 and E18.5 pancreas)

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The transcriptional repressor REST is a major suppressor of endocrine differentiation during pancreas organogenesis.

Project description:The transcriptional repressor REST is a major suppressor of endocrine differentiation during pancreas organogenesis.

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The transcriptional repressor REST is a major suppressor of endocrine differentiation during pancreas organogenesis [ChIP-seq]

Project description:The transcriptional repressor REST is a major suppressor of endocrine differentiation during pancreas organogenesis [ChIP-seq]

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The transcriptional repressor REST is a major suppressor of endocrine differentiation during pancreas organogenesis [RNA-seq]

Project description:The transcriptional repressor REST is a major suppressor of endocrine differentiation during pancreas organogenesis [RNA-seq]

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REST is a major negative regulator of endocrine differentiation during pancreas organogenesis.

Project description:Multiple transcription factors have been shown to promote pancreatic β-cell differentiation, yet much less is known about negative regulators. Earlier epigenomic studies suggested that the transcriptional repressor REST could be a suppressor of endocrinogenesis in the embryonic pancreas. However, pancreatic Rest knockout mice failed to show abnormal numbers of endocrine cells, suggesting that REST is not a major regulator of endocrine differentiation. Using a different conditional allele that enables profound REST inactivation, we observed a marked increase in pancreatic endocrine cell formation. REST inhibition also promoted endocrinogenesis in zebrafish and mouse early postnatal ducts and induced β-cell-specific genes in human adult duct-derived organoids. We also defined genomic sites that are bound and repressed by REST in the embryonic pancreas. Our findings show that REST-dependent inhibition ensures a balanced production of endocrine cells from embryonic pancreatic progenitors.

| S-EPMC8415321 | biostudies-literature

The transcriptional repressor REST is a major suppressor of endocrine differentiation during pancreas organogenesis.

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-06-30 | GSE179120 | GEO

e18.5 pancreas: WT vs Cyp11a1 KO

Project description:RNA profiling of WT and cyp11a1 KO pancreas at e18.5

2021-06-25 | GSE178633 | GEO

e18.5 pancreas: WT vs Cyp11a1 KO

Project description:e18.5 pancreas: WT vs Cyp11a1 KO

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TrxG complex catalytic and non-catalytic activity play distinct roles in pancreas progenitor specification and differentiation (E18.5 Dpy30∆P pancreas)

Project description:Purpose: To gain insight into why Dpy30∆P acinar cells fail to express terminal makers, we performed RNA-seq using E18.5 control and Dpy30∆P pancreata. Methods: Floxed Dpy30 mice were crossed to Pdx1-Cre driver mice to obtain conditional deletion of Dpy30 exon 4 in the pancreas. In all studies, knockout mice (Dpy30∆P, Pdx1-Cre; Dpy30flox/flox) were compared to littermate controls (Dpy30flox/flox or Dpy30flox/wt). Results: Differential gene expression analysis demonstrated that endocrine cell hormones such as Ins2 and acinar cell digestive enzymes were downregulated in the E18.5 Dpy30∆P pancreas compared to controls. GO term analysis indicated that genes involved in pancreatic secretion, digestion and proteolysis were reduced in Dpy30∆P cells. Notably, transcripts with very high expression levels (above 9000 FPKM) were more downregulated in the E18.5 Dpy30∆P pancreas compared to transcripts with lower expression levels. Conclusions: Overall, these data suggest increased variation in acinar cell transcript expression in the Dpy30∆P pancreas.

2019-07-12 | GSE134180 | GEO

REST–Mediated Recruitment of Polycomb Repressor Complexes in Mammalian Cells

Project description:Genome-wide analysis of REST and Polycomb binding in Rest-/- and Eed-/- mouse embryonic stem (mES) cells showed that Rest was required for PRC1 recruitment to a subset of Polycomb regulated neuronal genes. Furthermore, we found that PRC1 can be recruited to Rest binding sites independently of CpG islands and the H3K27Me3 mark., and that PRC2 was frequently increased around Rest binding sites located in CpG-rich regions in the Rest2/2 mES cells. Published here: PLoS Genet 8(3): e1002494. doi:10.1371/journal.pgen.1002494

2013-06-20 | GSE48122 | GEO

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