Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Next-generation sequencing measuring genome-wide RNA decay by s4U Seq in naïve CD4+ T cells from Mettl3 WT and KO mice

ABSTRACT: Naïve T cells of Mettl3 KO and WT were pulse labeled by s4U for 15 min before and after IL-7 stimulation for 15min/30min/45min/60min/75min/90min. The total input RNAs or s4U nascent RNAs were isolated and prepared for next-generation sequencing. The RNA response to IL-7 and RNA decay rates were then modeled and calculated, to infer the m6A/Mettl3 effects.

ORGANISM(S): Mus musculus

PROVIDER: GSE100278 | GEO | 2017/08/17

SECONDARY ACCESSION(S): PRJNA391351

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Targeted transcriptome analysis of the SigB regulon in Bacillus subtilis

Project description:Bacillus subtilis is exposed to a wide range of transitory stress and starvation conditions. Here we investigate the expression changes observed in the B. subtilis wild type strain 168 and its isogenic sigB mutant(BSM29) with respect to each stress condition tested. Gene expression was queried for the stress conditions: ethanol-, butanol-, osmotic- and oxidative stress, heat shock, low temperature growth, glucose as well as oxygen limitation. For butanol-, ethanol-, osmotic-, and oxidative stress as well as heat shock : time points (0min, 5min, 10min, 15min and 20min) ; for glucose limitation and oxygen limitation : time points (0min, 15min, 30min, 45min, 60min or 90min) and for low temperature growth, samples for recording of expression values were taken during mid-exponential growth at OD540 0.9 and 1.0.

2012-03-14 | E-GEOD-32895 | biostudies-arrayexpress

Transcriptomic profiles of D29, StarStuff and L5

Project description:The gene expression profiles of mycobacteriophage D29 and StarStuff were analyzed 15min, 30min, 60min and 150min post-infection (MOI=3), as well as a StarStuff lysogen. The gene expression profiles of 30min and 150min post-induction of an L5 thermo-inducible lysogen, as well as the L5 lysogen itself.

2017-11-13 | GSE99182 | GEO

Directional RNA-seq reveals highly complex condition-dependent transcriptomes in E. coliK12 through accurate full-length transcripts assembling

Project description:Using a highly efficient and strand-specific RNA-seq method combined with a highly accurate and robust algorithm and tool we developed, TruHMM for assembling full-length transcriptomes, to profile the transcriptome of E.coli K12 under different culture conditions and growth phases, we showed that the dynamic transcriptome structures appears to be culture condition and growth phases dependent. mRNA profiles of E.coli K12 cultured under different treaments including LB (OD=0.87), Heatshock (15min, 30min, 60min), and MOPS-P(0h,2h,4h).

2013-06-21 | E-GEOD-48151 | biostudies-arrayexpress

Transcription profiling time series of Arabidopsis seedlings treated with the cytokinin benzyladenine or DMSO

Project description:10 day old seedlings were treated with 5uM of the cytokinin Benzyladenine(BA)or DMSO at 15min, 45min, 120min, 480min and 1440min

2006-08-24 | E-MEXP-749 | biostudies-arrayexpress

Radicicol treatment of Drosophila cells

Project description:Drosophila S2 cells were treated with Heat-shock protein 90 (Hsp90) inhibitor radicicol for 15min, 30min and 1h. Poly(A) RNA was isolated and sequenced.

2012-05-15 | GSE35812 | GEO

Short enzymatic reactions in proteomics studies

Project description:SKBR3 breast cancer cell extracts were digested with trypsin (or LysC) on short time scales (7min, 15min, 30min, 1h, and 18h), and the results were compared to overnight digestion protocols.

2018-09-24 | PXD010306 | Pride

The Tec kinase ITK optimizes differential NFAT, NF-κB, and Erk1/2 signaling to regulate graded gene induction in mouse CD8+ T cells [ATAC-seq]

Project description:In this study we generated ATAC-seq libraries from mouse CD8+ OT-I T cells that were stimulated with WT splenocytes loaded with OVA peptide or OVA peptide variants, with or without presence of ITK inhibitor PRN-694 at early time points (0, 30min, 60min, or 120min).

2021-02-23 | GSE167302 | GEO

Snf1 is involved in the aberrant cell cycle induction in cdc14-3 mutant upon NaCl stress in S. cerevisiae

Project description:Transcriptome profiling of cdc14-3 and cdc14-3_snf1 mutants at non-permissive temp (35C) for 90min or 2hr followed by 30min NaCl stress. The experiment showed the aberrant cell cycle induction in cdc14-3 mutants upon NaCl response is abrogated with snf1 deletion in cdc14-3 background.

2014-12-03 | GSE56995 | GEO

The Tec kinase ITK optimizes differential NFAT, NF-κB, and Erk1/2 signaling to regulate graded gene induction in mouse CD8+ T cells [RNA-seq]

Project description:In this study we generated SMART-seq cDNA libraries from mouse CD8+ OT-I T cells that were stimulated with WT splenocytes loaded with OVA peptide or OVA peptide variants, with or without presence of ITK inhibitor PRN-694 at early time points (0, 30min, 60min, or 120min).

2021-02-23 | GSE167303 | GEO

Radicicol treatment of Drosophila cells

Project description:Drosophila S2 cells were treated with Heat-shock protein 90 (Hsp90) inhibitor radicicol for 15min, 30min and 1h. Poly(A) RNA was isolated and sequenced. Kinetics of transcriptional response to Hsp90 inhibition

2012-05-14 | E-GEOD-35812 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data