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Radicicol treatment of Drosophila cells

ABSTRACT: Drosophila S2 cells were treated with Heat-shock protein 90 (Hsp90) inhibitor radicicol for 15min, 30min and 1h. Poly(A) RNA was isolated and sequenced. Kinetics of transcriptional response to Hsp90 inhibition

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Cem Sievers

PROVIDER: E-GEOD-35812 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Hsp90 globally targets paused RNA polymerase to regulate gene expression in response to environmental stimuli.

Sawarkar Ritwick R Sievers Cem C Paro Renato R

Cell 20120501 4

The molecular chaperone Heat shock protein 90 (Hsp90) promotes the maturation of several important proteins and plays a key role in development, cancer progression, and evolutionary diversification. By mapping chromatin-binding sites of Hsp90 at high resolution across the Drosophila genome, we uncover an unexpected mechanism by which Hsp90 orchestrates cellular physiology. It localizes near promoters of many coding and noncoding genes including microRNAs. Using computational and biochemical anal ...[more]

PMID: 22579285

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