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Transcriptome analysis using RNA sequencing conducted for wild type (Col-0), ahl10-1 and rrp6l1-2 mutant under control conditions and after 96 h at moderate low water potential (-0.7 MPa)

ABSTRACT: RNA sequencing was used to identify genes differentially expressed (DEG) in ahl10-1 mutants and rrp6l1-2 mutant compared to Col-0 wild type. Two treatments were analyzed for each genotype: unstressed control and a 96 hour low water potential (-0.7 MPa) treatment. Approximately 2000-3000 genes were found to be significantly up and down-regulated in the wild type stress versus control treatment using . rrp6l1-2 mutant shows around 500 and 250 genes up and down regulated respectively in control treated seedlings while 10 or less than 10 found in ahl10-1. In the stress treatment, rrp6l1-2 and ahl10-1 have less and 60 genes up and downregulated. ahl10-1 and rrp6l-2 have a common set of downregulated genes (24 genes) at stress treatment.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE101197 | GEO | 2022/07/15

REPOSITORIES: GEO

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Project description:Gene expression was measured in control and heat resistance selected adult female flies before and at 8 time points after heat stress for 1h @ 36 degrees,Abstract The availability of full genome sequences has allowed the construction of microarrays, with which screening,of the full genome for changes in gene expression is possible. This method can provide a wealth of information about,biology at the level of gene expression and is a powerful method to identify genes and pathways involved in various,processes. In this study, we report a detailed analysis of the full heat stress response in Drosophila melanogaster,females, using whole genome gene expression arrays (Affymetrix Inc, Santa Clara, CA, USA). The study focuses on,up- as well as downregulation of genes from just before and at 8 time points after an application of short heat hardening,(368C for 1 hour). The expression changes were followed up to 64 hours after the heat stress, using 4 biological,replicates. This study describes in detail the dramatic change in gene expression over time induced by a short-term,heat treatment. We found both known stress responding genes and new candidate genes, and processes to be involved,in the stress response. We identified 3 main groups of stress responsive genes that were earlyâ??upregulated, earlyâ??,downregulated, and lateâ??upregulated, respectively, among 1222 differentially expressed genes in the data set. Comparisons,with stress sensitive genes identified by studies of responses to other types of stress allow the discussion of,heat-specific and general stress responses in Drosophila. Several unexpected features were revealed by this analysis,,which suggests that novel pathways and mechanisms are involved in the responses to heat stress and to stress in,general. The majority of stress responsive genes identified in this and other studies were downregulated, and the,degree of overlap among downregulated genes was relatively high, whereas genes responding by upregulation to heat,and other stress factors were more specific to the stress applied or to the conditions of the particular study. As an,expected exception, heat shock genes were generally found to be upregulated by stress in general.

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Transcriptome analysis using RNA sequencing conducted for wild type (Col-0), ahl10-1 and rrp6l1-2 mutant under control conditions and after 96 h at moderate low water potential (-0.7 MPa)

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