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Rrp6l1-2 project

ABSTRACT: 1, Using mRNA-Seq to get expression profiling of rrp6l1-2 mutant and Col-0 wild-type (WT); 2,Using MethylC-Seq to provide single-base resolution of DNA methylation status in rrp6l1-2 mutant; 3, Using small RNA-Seq(sRNA-Seq) to get small RNA profiling of rrp6l1-2 and WT mRNA-Seq: 2 samples examined, WT and rrp6l1-2 mutant; MethylC-Seq: 1 sample examined, rrp6l1-2 mutant; small RNA-Seq: 2 samples examined, WT and rrp6l1-2 mutant

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Jian-Kang Zhu

PROVIDER: E-GEOD-50486 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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An Rrp6-like protein positively regulates noncoding RNA levels and DNA methylation in Arabidopsis.

Zhang Huiming H Tang Kai K Qian Weiqiang W Duan Cheng-Guo CG Wang Bangshing B Zhang Heng H Wang Pengcheng P Zhu Xiaohong X Lang Zhaobo Z Yang Yu Y Zhu Jian-Kang JK

Molecular cell 20140410 3

Rrp6-mediated nuclear RNA surveillance tunes eukaryotic transcriptomes through noncoding RNA degradation and mRNA quality control, including exosomal RNA decay and transcript retention triggered by defective RNA processing. It is unclear whether Rrp6 can positively regulate noncoding RNAs and whether RNA retention occurs in normal cells. Here we report that AtRRP6L1, an Arabidopsis Rrp6-like protein, controls RNA-directed DNA methylation through positive regulation of noncoding RNAs. Discovered ...[more]

PMID: 24726328

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