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Transcriptome involved in T3 treated Japanese flounder (Paralichthys olivaceus)

ABSTRACT: After comparing albinism rate, T3 treatment and control larvae of 42 DAH were chosen for next high-throughput sequencing analyses of mRNA. RNA-seq identified 146 differentially expressed genes (DEGs) in D42_T3 versus D42_Con (|log2Foldchange| > 1 and q < 0.005), including pigmentation related genes such as the receptor tyrosine-protein kinase erbB-3, pro-opiomelanocortin A, melanotransferrin, and the growth-related gene somatotropin. These DEGs were significantly enriched to 15 GO terms and 8 KEGG pathways (q < 0.05), among which, several sugar metabolic pathways were included (glycolysis/gluconeogenesis and the pentose phosphate pathway).

ORGANISM(S): Paralichthys olivaceus

PROVIDER: GSE104918 | GEO | 2019/12/31

REPOSITORIES: GEO

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microRNA data involved in T3 treated Japanese flounder (Paralichthys olivaceus)

Project description:After comparing albinism rate, T3 treatment and control larvae of 42 DAH were chosen for next high-throughput sequencing analyses of miRNA. A total of 337 miRNAs were identified via miRNA-seq and 12 miRNAs exhibited significantly differential expression patterns in D42_T3 vs D42_Con (TPM > 10, fold change ≥ 1.5 or ≤ 0.67 and q ≤ 0.05). These differentially expressed miRNAs targeted 3658 putative genes, further enriching 10 GO terms (q < 0.05).

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microRNA data involved in Japanese flounder (Paralichthys olivaceus) albinism

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Transcriptome data involved in Japanese flounder (Paralichthys olivaceus) albinism

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Project description:Size-scaling metabolism is widely considered to be of significant importance in biology and ecology. Thus, allometric relationships between metabolic rate (VO2) and body mass (M), VO2 = a(i)Mb, have long been a topic of interest and speculation. It has been proposed that intraspecifically metabolic rate scales isometrically or near isometrically with body mass during the early life history in fishes, invertebrates, birds and mammals. We developed a new perspective on intraspecific size-scaling metabolism through determination of metabolic rate in the Japanese flounder, Paralichthys olivaceus, during their early life stages spanning approximately four orders of magnitude in body mass. With the increase of body mass, the Japanese flounder had four distinct negative allometric phases in which three stepwise increases in scaling constants (ai, i = 1-4), i.e. ontogenetic phase shifts in metabolism, occurred with growth during its early life stages at around 0.002, 0.01 and 0.2 g, maintaining each scaling exponent constant in each phase (b = 0.831). These shifts in metabolism during the early life stages are similar to the tiger puffer, Takifugu rubripes. Our results indicate that ontogenetic phase shifts in metabolism are key to understanding intraspecific size-scaling metabolism in fishes.

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Paralichthys olivaceus

Project description:Transcriptome data involved in Japanese flounder (Paralichthys olivaceus) albinism

| PRJNA388164 | ENA

Paralichthys olivaceus

Project description:microRNA data involved in Japanese flounder (Paralichthys olivaceus) albinism

| PRJNA388163 | ENA

Transcriptional responses of olive flounder (Paralichthys olivaceus) to low temperature.

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