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MicroRNA data involved in T3 treated Japanese flounder (Paralichthys olivaceus)

ABSTRACT: After comparing albinism rate, T3 treatment and control larvae of 42 DAH were chosen for next high-throughput sequencing analyses of miRNA. A total of 337 miRNAs were identified via miRNA-seq and 12 miRNAs exhibited significantly differential expression patterns in D42_T3 vs D42_Con (TPM > 10, fold change ≥ 1.5 or ≤ 0.67 and q ≤ 0.05). These differentially expressed miRNAs targeted 3658 putative genes, further enriching 10 GO terms (q < 0.05).

ORGANISM(S): Paralichthys olivaceus

PROVIDER: GSE104919 | GEO | 2019/12/31

REPOSITORIES: GEO

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Transcriptome involved in T3 treated Japanese flounder (Paralichthys olivaceus)

Project description:After comparing albinism rate, T3 treatment and control larvae of 42 DAH were chosen for next high-throughput sequencing analyses of mRNA. RNA-seq identified 146 differentially expressed genes (DEGs) in D42_T3 versus D42_Con (|log2Foldchange| > 1 and q < 0.005), including pigmentation related genes such as the receptor tyrosine-protein kinase erbB-3, pro-opiomelanocortin A, melanotransferrin, and the growth-related gene somatotropin. These DEGs were significantly enriched to 15 GO terms and 8 KEGG pathways (q < 0.05), among which, several sugar metabolic pathways were included (glycolysis/gluconeogenesis and the pentose phosphate pathway).

2019-12-31 | GSE104918 | GEO

microRNA data involved in Japanese flounder (Paralichthys olivaceus) albinism

Project description:In this study, a high-throughput sequencing strategy was employed to identify the miRNAs involved in P. olivaceus albinism. High-throughput miRNA sequencing identified a total of 475 miRNAs, including 64 novel miRNAs. Furthermore, 33 differentially expressed miRNAs containing 13 up-regulated and 20 down-regulated miRNAs were identified in albino versus normally pigmented individuals (fold change ≥1.5 or ≤0.67 and p≤0.05).

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Transcriptome data involved in Japanese flounder (Paralichthys olivaceus) albinism

Project description:In this study, a high-throughput sequencing strategy was employed to identify the mRNA involved in P. olivaceus albinism. Based on P. olivaceus genome, RNA-seq identified 21,787 know genes and 711 new genes by transcripts assembly. Of those, 235 genes exhibited significantly different expression pattern (fold change ≥2 or ≤0.5 and q-value≤0.05), including 194 down-regulated genes and 41 up-regulated genes in albino versus normally pigmented individuals. These genes were enriched to 81 GO terms and 9 KEGG pathways (p≤0.05). Among those, the pigmentation related pathways-Melanogenesis and tyrosine metabolism were contained.

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Ontogenetic phase shifts in metabolism in a flounder Paralichthys olivaceus.

Project description:Size-scaling metabolism is widely considered to be of significant importance in biology and ecology. Thus, allometric relationships between metabolic rate (VO2) and body mass (M), VO2 = a(i)Mb, have long been a topic of interest and speculation. It has been proposed that intraspecifically metabolic rate scales isometrically or near isometrically with body mass during the early life history in fishes, invertebrates, birds and mammals. We developed a new perspective on intraspecific size-scaling metabolism through determination of metabolic rate in the Japanese flounder, Paralichthys olivaceus, during their early life stages spanning approximately four orders of magnitude in body mass. With the increase of body mass, the Japanese flounder had four distinct negative allometric phases in which three stepwise increases in scaling constants (ai, i = 1-4), i.e. ontogenetic phase shifts in metabolism, occurred with growth during its early life stages at around 0.002, 0.01 and 0.2 g, maintaining each scaling exponent constant in each phase (b = 0.831). These shifts in metabolism during the early life stages are similar to the tiger puffer, Takifugu rubripes. Our results indicate that ontogenetic phase shifts in metabolism are key to understanding intraspecific size-scaling metabolism in fishes.

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Identification and differential expression of microRNAs during metamorphosis in the Japanese flounder (Paralichthys olivaceus)

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Identification and differential expression of microRNAs during metamorphosis in the Japanese flounder (Paralichthys olivaceus)

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Project description:microRNA data involved in Japanese flounder (Paralichthys olivaceus) albinism

| PRJNA388163 | ENA

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Project description:Transcriptome data involved in Japanese flounder (Paralichthys olivaceus) albinism

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Transcriptional responses of olive flounder (Paralichthys olivaceus) to low temperature.

Project description:The olive flounder (Paralichthys olivaceus) is an economically important flatfish in marine aquaculture with a broad thermal tolerance ranging from 14 to 23°C. Cold-tolerant flounder that can survive during the winter season at a temperature of less than 14°C might facilitate the understanding of the mechanisms underlying the response to cold stress. In this study, the transcriptional response of flounder to cold stress (0.7±0.05°C) was characterized using RNA sequencing. Transcriptome sequencing was performed using the Illumina MiSeq platform for the cold-tolerant (CT) group, which survived under the cold stress; the cold-sensitive (CS) group, which could barely survive at the low temperature; and control group, which was not subjected to cold treatment. In all, 29,021 unigenes were generated. Compared with the unigene expression profile of the control group, 410 unigenes were up-regulated and 255 unigenes were down-regulated in the CT group, whereas 593 unigenes were up-regulated and 289 unigenes were down-regulated in the CS group. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses revealed that signal transduction, lipid metabolism, digestive system, and signaling molecules and interaction were the most highly enriched pathways for the genes that were differentially expressed under cold stress. All these pathways could be assigned to the following four biological functions for flounder that can survive under cold stress: signal response to cold stress, cell repair/regeneration, energy production, and cell membrane construction and fluidity.

| S-EPMC4184807 | biostudies-literature

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