ABSTRACT: Gene Expression profiling of the Arabidopsis thaliana MAP Kinase Kinases 1 (mkk1), MAP Kinase Kinases 2 (mkk2) knockout mutants and the double mutant mkk1/mkk2 before and 24 hours after treatment with the salicylic acid analog BTH, was measured by hybridisation to an Affymetrix ATH1 GeneChip. Keywords: Gene expression. Combined mutant and treatment study
Project description:Gene Expression profiling of the Arabidopsis thaliana MAP Kinase Kinases 1 (mkk1), MAP Kinase Kinases 2 (mkk2) knockout mutants and the double mutant mkk1/mkk2 before and 24 hours after treatment with the salicylic acid analog BTH, was measured by hybridisation to an Affymetrix ATH1 GeneChip. Experiment Overall Design: Whole Arabidopsis thaliana mkk1, mkk2 and mkk1/mkk2 knockout mutants and wild type plants (Col-0) were sampled in triplicates before and 24 hours after BTH treatment.
Project description:au08-02_mkk1-mkk2 - mkk1-mkk2 - Functional redundancy of the two MAPKKs MKK1 and MKK2. - mkk1 and mkk2 k.o. plants were crossed. Seedlings homozygous for mkk1 and/or mkk2, in parallel with control wild type seedlings, were grown on sterile medium. RNA was extracted from non-treated seedlings. aRNA was synthesised using the Ambion aRNA kit. Transcriptome data will be compared with those from a (already available) microarray of a mutant lacking the MAPKKkinase that is upstream of MKK1 and MKK2 (MEKK1).
Project description:au08-02_mkk1-mkk2 - mkk1-mkk2 - Functional redundancy of the two MAPKKs MKK1 and MKK2. - mkk1 and mkk2 k.o. plants were crossed. Seedlings homozygous for mkk1 and/or mkk2, in parallel with control wild type seedlings, were grown on sterile medium. RNA was extracted from non-treated seedlings. aRNA was synthesised using the Ambion aRNA kit. Transcriptome data will be compared with those from a (already available) microarray of a mutant lacking the MAPKKkinase that is upstream of MKK1 and MKK2 (MEKK1). 4 dye-swap - gene knock out
Project description:Wind is one of the most prevalent environmental forces entraining plants to develop various mechano-responses, collectively called thigmomorphogenesis. Largely unknown is how plants transduce the complex wind force signals downstream to nuclear events and the development of thigmomorphogenic phenotype or anemotropic response. To identify molecular components of the wind drag force signaling, two force-regulated phosphoproteins, identified from our previous phosphoproteomic study of Arabidopsis touch response, mitogen-activated protein kinase kinase 1 (MKK1) and 2 (MKK2), were selected for performing in planta TurboID (ID)-based proximity-labeling (PL) proteomics. This quantitative biotinylproteomics was separately performed on MKK1-ID and MKK2-ID transgenic plants, respectively, using the TurboID overexpression transgenics as a universal control. This quantitative biotinylproteomic work successfully identified 11 and 71 MKK1- and MKK2 - associated proteins, respectively. A WInd-Related Kinase 1 (WIRK1), previously known as Rapidly Accelerated Fibrosarcoma 36 protein (RAF36), was eventually found to be a common interactor for both MKK1 and MKK2 kinases. Further molecular biology studies of the Arabidopsis RAF36 kinase found that it plays a role in wind regulation of the expression of touch-responsive TCH3 and CML38 genes and the phosphorylation of a touch-regulated PATL3 phosphoprotein. Measurement of leaf morphology and shoot gravitropic response of wirk1-1 mutant revealed that the WIRK1 gene is involved in both wind response and gravitropism of Arabidopsis, suggesting that WIRK1 protein may serve as the crosstalk point among multiple signal transduction pathways of both gravitropic and wind responses. It is likely that gravity force signaling may be an integral part of the wind mechano-signaling network in various parts of plant organs.
Project description:adt06-03-mkk2 - mkk2_mkk2_and_wrky19_cold_time_course - early response targets of MKK2 upon cold stress treatment and its role in adaptation. - seedlings were put to 4 ° comparing wild type ColO versus mkk2 knockouts, MKK2 overexpressors and wrky19 knockouts. Keywords: time course,wt vs mutant comparison
Project description:Benzothiadiazole (BTH) is a so-called ‘plant activator’ and protects plants from diseases by activating the salicylic-acid (SA) signaling pathway. We identified BTH-responsive genes in rice leaves 24 h after treatment using rice 44K microarray. Keywords: response to chemical treatment
Project description:The project quantified the yeast phosphoproteome before and at various times after dithiolthreitol (DTT) in budding yeast, focusing on wild-type cells and mutants laking the Ire1 and Mkk1/Mkk2 kinases.
Project description:adt06-03-mkk2 - mutants - early response targets of MKK2 upon cold stress treatment and its role in adaptation. - Several mutants in MKK pathway were tested against the wild type. Keywords: gene knock out