Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression patterns in Normal Human Epidermal Melanocytes at different time points

ABSTRACT: Normal human epidermal melanocytes (NHEM, purchased from PromoCell) were culture for one (7 days) or two weeks (14 days) and subsequently, total RNA was isolated and cDNA array analysis was performed. The array was used to determine general gene expression patterns in primary melanocytes. Moreover, gene expression alterations during cell culturing (one or two weeks) can be detected. Finally, the gene expression array is used to compared gene expression paterns with melanoma cell and tissue gene expression patterns or with other primary cell lines.

ORGANISM(S): Homo sapiens

PROVIDER: GSE108969 | GEO | 2020/11/16

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

MiRNA-sequencing in normal human epidermal melanocytes, melanoblast-related cells and melanoma cell lines

Project description:The study aims to identify differences in miRNA expression during melanoma development. Therefore, normal human epidermal melanocytes (NHEM) were de-differentiated into melanoblast-related cells (MBrCs) to model processes during embryonic differentiation and to compare them to the tumorigenic phenotype in melanoma development.

2021-05-26 | GSE174334 | GEO

total RNA-seq from melanocyte of skin (ENCSR000CUR)

Project description:RNA-seq on human skin melanocytes primary whole cells (NHEM-M2) rRNA-depleted Total RNA greater than 200 nucleotides in size. (paired end) For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2016-03-18 | GSE78619 | GEO

total RNA-seq from melanocyte of skin (ENCSR000CUQ)

Project description:RNA-seq on humn skin melanocytes primary whole cells (NHEM-M2) from rRNA-depleted total RNA greater than 200 nucleotides in size. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2016-03-18 | GSE78618 | GEO

Effect of CXCL1 and CXCL1 with SX-682 on gene expression for human epidermal melanocytes (NHEM) cells.

Project description:Effect of CXCL1 and CXCL1 with SX-682 on gene expression for human epidermal melanocytes (NHEM) cells.

| PRJNA925525 | ENA

RNA-sequencing of melanoma celllines compared to normal human melanocytes (NHEMs)

Project description:Given that melanomas gain different properties during their progression, 6 different melanoma cell lines (Sbcl-2, WM3211, WM793, WM1366, WM1158, WM9) were selected and categorized by tumor stage (primary tumor (PT), metastasis (MET)) as well as the previously described BRAF and PTEN mutation status. Then, differentially expressed genes in malignant melanoma were identified by performing RNA-Sequencing (RNA-seq) with all aforementioned melanoma cell lines and normal human epidermal melanocytes (NHEM).

2024-12-20 | GSE284779 | GEO

Transcription profiling of Pseudomonas aeruginosa strains 383 and 2192

Project description:At mid-log phase (OD600 of 0.5), unique gene expression patterns were observed between these two strains with 3.4% of the transcripts (188/5570) expressed differentially. Of the 188 significantly varied (>1.8 fold) genes, 115 were up-regulated in 383 while 73 were up-regulated in 2192. Experiment Overall Design: The goal of this experiment was to identify the differentially expressed genes from two genetically similar but phenotypically distinct P. aeruginosa strains 383 and 2192. Two strains were isolated two days apart from the sputum of the same cystic fibrosis patient. Following proper culture RNA was extracted from the two strains. Affymetrix GeneChip Pseudomonas aeruginosa was used to examine the gene expression paterns of the two strains.

2008-06-16 | E-GEOD-9621 | biostudies-arrayexpress

Genomics of the ovine heart ontogeny during the perinatal period

Project description:The period of development from the last two weeks of gestation through the first two weeks of life spans a period of great functional and metabolic challenge to the fetal and neonatal lamb heart. Important changes in gene expression occur to meet these challenges. On this study, septa from sheep hearts at 130 days gestation (n=6), term (n=8, gestational lenght is around 145 days) and 14-days-old lambs (n=8) were used to model the changes in gene expression patterns during the perinatal period using Agilent 15k ovine microarrays. Weighted gene co-expression network analysis (WGCNA) determined five major patterns of co-expressed and functionally related genes during this critical period of cardiac transition.

2015-07-30 | GSE66725 | GEO

Clonal competition with alternating dominance in multiple myeloma [244kCGH]

Project description:Copy number and Gene expression profiling multiple myeloma patients at multiple stages of their individual clinical course Identification of evolutionary paterns in multiple myeloma

2012-03-28 | GSE36822 | GEO

Clonal competition with alternating dominance in multiple myeloma [44kCGH]

2012-03-28 | GSE36823 | GEO

Gene expression patterns in Normal Human Epidermal Melanocytes at different time points

Project description:Gene expression patterns in Normal Human Epidermal Melanocytes at different time points

| PRJNA429260 | ENA