Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Analysis of pathways elicited by empty SV40 capsids (VLPs) in septic rats 6 hours post 2CLP operation

ABSTRACT: During the first hours after infection, before SV40 DNA enters the nucleus, empty capsids (VLPs) and the wild-type virus have the same effect on cellular signaling. VLPs were previously found to ameliorate toxic acute kidney injury (AKI) in a mouse model, counteracting apoptosis by inducing the Akt-1 survival pathway. Here we tested their effect on severe sepsis in rats. VLP pre-treatment increased survival and recovery from zero to 75%. RNAseq studies demonstrated that unlike AKI, here the VLPs did not induce survival pathways. Instead they affected thousands of genes and many cellular functions, eliminating deleterious pathways and inducing beneficial ones: immune response, resolution of inflammation, regeneration and cell and system homeostasis. In contrast, only four genes were affected following VLP administration to healthy rats. We propose that SV40 VLPs respond specifically to perturbation in cellular activities. The study suggests that diseases with complex pathophysiology may require treatments affecting wide-spectrum functions.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE109041 | GEO | 2020/03/02

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Analysis of pathways elicited by empty SV40 capsids (VLPs) in septic rats 24 hours post 2CLP operation

Project description:During the first hours after infection, before SV40 DNA enters the nucleus, empty capsids (VLPs) and the wild-type virus have the same effect on cellular signaling. VLPs were previously found to ameliorate toxic acute kidney injury (AKI) in a mouse model, counteracting apoptosis by inducing the Akt-1 survival pathway. Here we tested their effect on severe sepsis in rats. VLP pre-treatment increased survival and recovery from zero to 75%. RNAseq studies demonstrated that unlike AKI, here the VLPs did not induce survival pathways. Instead they affected thousands of genes and many cellular functions, eliminating deleterious pathways and inducing beneficial ones: immune response, resolution of inflammation, regeneration and cell and system homeostasis. In contrast, only four genes were affected following VLP administration to healthy rats. We propose that SV40 VLPs respond specifically to perturbation in cellular activities. The study suggests that diseases with complex pathophysiology may require treatments affecting wide-spectrum functions.

2020-03-02 | GSE125276 | GEO

Empty SV40 capsids (VLPs) significantly improve survival and recovery of septic rats

Project description:Empty SV40 capsids (VLPs) significantly improve survival and recovery of septic rats

| PRJNA515732 | ENA

RNA Sequencing of dendritic cells treated with glycan-costumed virus-like particles

Project description:We engineered a glycan-costumed virus-like particle (VLP) vaccine that delivers programmable peptide antigens to induce tumor-specific cellular immunity in vivo. VLPs encapsulating TLR7 agonists were decorated with a synthetic mannose-derived ligand (VLP-Man-OvaI/II) that selectively engages the lectin DC-SIGN. Lectin-TLR7 dual engagement induced robust DC activation and type 1 cellular immunity, whereas VLPs lacking this key DC-SIGN ligand (VLP-PE-OvaI/II) failed to promote DC-mediated cellular responses. We performed bulk RNA-seq experiments on moDCs treated with VLP-Man-OvaI/II and VLP-PE-OvaI/II or unstimulated control moDCs. A total of 486 genes were differentially expressed after treatment with VLP-Man-OvaI/II vs. VLP-PE-OvaI/II (log2FC >1.5 and p<0.05). The significantly upregulated genes included CXCL1, CXCL5, ISG15, IL6, CCL4, ISG20, and SOCS3, all of which are implicated in the TLR7 downstream signaling pathway, suggesting a higher extent of TLR7 activation with the VLP-Man-OvaI/II. Gene set enrichment analysis (GSEA) between VLP-Man-OvaI/II- and VLP-PE-OvaI/II-treated moDCs revealed that hallmark DC activation pathways were also upregulated in VLP-Man-OvaI/II-treated DCs, including pathways related to IFN-a, IFN-g, and TNF-a signaling via nuclear factor-κB (NF-kB). These data give insight into how lectin binding with glycan-costumed VLPs can be employed to reprogram immunity.

2024-09-06 | GSE251818 | GEO

Analysis of pathways elicited by empty SV40 capsids (VLPs) in septic rats 6 hours post 2CLP operation

Project description:Analysis of pathways elicited by empty SV40 capsids (VLPs) in septic rats 6 hours post 2CLP operation

| PRJNA429471 | ENA

Analysis of pathways elicited by empty SV40 capsids (VLPs) in septic rats 24 hours post 2CLP operation

Project description:Analysis of pathways elicited by empty SV40 capsids (VLPs) in septic rats 24 hours post 2CLP operation

| PRJNA515734 | ENA

Transcriptome analysis of dendritic cells activated by VLPs or ncRNAVLPs

Project description:Analysis of the effect on dendritic cells of the injection in C57BL/6 mice of VLPs or ncRNAVLPs at gene expression level.

2016-06-02 | GSE70557 | GEO

Arabidopsis retrotransposon virus-like-particles and their regulation by epigenetically activated small RNA

Project description:We report polysomal RNA sequencing data (RNAseq), small RNAseq, and virus-like-particle (VLP) DNA sequencing (DNAseq) and ChIPseq data. Arabidopsis ddm1 mutants produce LTR retrotransposon transcripts that are processed into 21-22 nt easiRNAs by RNA-Dependent RNA polymerase 6 (RDR6). To test if 21-22 nt easiRNAs regulate transcription, translation and reverse transcription of LTR retrotransposons, we compared ddm1 and ddm1rdr6 in polysomal RNAseq, VLP DNAseq and ChIPseq data. We found a handful of ATHILA elements were differentially regulated in ddm1rdr6 for polysomal RNAseq, VLP DNAseq, and ChIPseq datasets. Using short read and long read technologies, we also profiled functional LTR retrotransposons that made full-length DNA by reverse transcription inside VLPs. Small RNAseq data were obtained in pollen and inflorescence tissues in wild-type and ddm1.

2020-03-13 | GSE128932 | GEO

Early transcriptional signature in murine cDCs post immunisation with VLPs containing TLR ligands

Project description:We have investigated the in vivo transcriptional signature in dendritic cells (DCs) from mice immunised with VLPs containing distinct classes of nucleic acid and correlated the expression patterns with the efficiency of induced T cell responses.

2019-07-13 | GSE134192 | GEO

Analysis of gradient purified immune suppressive particles from L. heterotoma NY parasitoid wasp

Project description:The composition and biotic nature of wasp VLPs has remained uncharacterized. VLP proteomics have contributed to a new understanding of their unusual organelle nature and mechanism of action in suppressing host defense responses.

2017-09-13 | PXD005639 | Pride

Analysis of gradient purified immune suppressive particles from L. heterotoma 14 parasitoid wasp

2017-09-13 | PXD005632 | Pride