Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide mapping of SRC-3 binding in the liver at CT4.

ABSTRACT: We reported the cistrome of steroid receptor co-activator SRC-3 in the liver at CT4

ORGANISM(S): Mus musculus

PROVIDER: GSE110462 | GEO | 2018/02/12

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

SRC-1 targets ADAM22: an ER-independent mechanism of tumour progression in endocrine resistance

Project description:We profile the binding of Steroid Receptor Co-activator (SRC1) in LY2 cells, a tamoxifen-resistant cell line, in the presence and absence of tamoxifen using ChIP-sequencing technology. The development of breast cancer resistance to endocrine therapy results from an increase in cellular plasticity leading to the development of a steroid-independent tumour. The p160 steroid coactivataor protein SRC-1, through interactions with developmental proteins and other non-steroidal transcription factors, drives this tumour adaptability. Here, using discovery studies, we identify ADAM22, a non-protease member of the ADAMs family, as a direct, ER-independent target of SRC-1. Molecular, cellular and in vivo studies confirmed SRC-1 as a regulator of ADAM22. At a functional level, a role for ADAM22 in cellular migration and differentiation was observed. In vivo data from a mouse xenograft model indicated that ADAM22 expression was higher in 4-OHT-treated endocrine-resistant tumours than in tumours derived from isogenic, sensitive cells. Furthermore, in breast cancer patients, ADAM22 expression is an independent predictor of poor disease free survival. SRC-1 can function as a molecular switch which converts a steroid-responsive tumour to a steroid-resistant tumour. The ER-independent SRC-1 target ADAM22 is a potential drug target and a companion predictive biomarker in the treatment of endocrine-resistant breast cancer. Examination of SRC-1 binding in LY2 cells in the presence or absence of tamoxifen treatment. 2 replicates each.

2011-11-01 | E-GEOD-28987 | biostudies-arrayexpress

Transcription array by profiling in WT and SRC-2 null mouse liver

Project description:The molecular targets of SRC-2 regulation in the murine liver stimulate fatty acid degradation and glycolytic pathway while fatty acid, cholesterol, and steroid biosynthetic pathways are down-regulated. A genomic approach using microarray analysis was employed to identify the subsets of genes that are altered in the liver of SRC-2-/- mice.

2014-02-14 | E-GEOD-55002 | biostudies-arrayexpress

SRC-1 targets ADAM22: an ER-independent mechanism of tumour progression in endocrine resistance

2011-11-01 | GSE28987 | GEO

Genome-wide mapping of SRC-3 binding in the liver at CT4.

Project description:Genome-wide mapping of SRC-3 binding in the liver at CT4.

| PRJNA433839 | ENA

Transcription array by profiling in WT and SRC-2 null mouse liver

2014-02-14 | GSE55002 | GEO

Time course SRC-3 ChIP-Seq and XBP1s ChIP-Seq in the C57BJ/L6 WT, SRC-3 WT versus KO, and Xbp1 WT versus LKO mice under chow and fasting conditions

Project description:We report the hepatic SRC-3 and XBP1s cistrome at 4-hour interval timecourse zeitgeber times (ZTs) in the liver of the C57BJ/L6 WT mice under chow and fasting conditions; we also chose liver samples at CT24, ZT0 and ZT8 for comprehensive SRC-3 and XBP1s ChIP-Seq to interrogate the genomic binding profiles of hepatic SRC-3 and XBP1s in vivo.

2021-12-31 | GSE175559 | GEO

Genome-wide Profiling of SRC-2 (NCOA2) binding in mouse prostate [ChIP-Seq]

Project description:Using genetically engineered mice, overexpressing SRC-2, specifically in the prostate epithelium of PTEN heterozygous mice accelerates PTEN mutation induce tumor progression and develops a metastasis-prone cancer. Here we used ChIP-Seq analysis to identify genome-wide SRC-2 binding sites in mouse prostate. Examination of genome-wide SRC-2 binding in mouse prostate by ChIP-seq analysis. Samples were collected from pooled dorsal-lateral prostates of 7 months old-PTEN flox/+; Rosa26-SRC-2 OE/+ mice. Flash frozen tissues were then sent to Active Motif, Inc. for chromatin extraction and followed by immunoprecipitation using anti-SRC-2 antibody (A300-346A, Bethyl Lab., Inc.).

2014-06-30 | E-GEOD-54770 | biostudies-arrayexpress

A SRC-3 gene signature in predecidual T-HESC

Project description:Steroid Receptor Coactivator-3 (SRC-3) knockdown in human endometrial stromal cells (HESCs) blocks their decidualization. This result provides translational support for recent studies in the mouse in which conditional SRC-3 knockout in progesterone receptor-positive cells of the endometrium results in early pregnancy loss due to a defect in normal decidualization. RNAseq was performed on the telomerase-immortalised endometrial stromal cell line T-HESC (CRL-4003; American Type Culture Collection) with or without SRC-3 knockdown to identify the transcriptome that is dependent on SRC-3 prior to hormone-dependent HESC decidualization.

2022-11-15 | GSE210936 | GEO

Genome-wide Profiling of Steroid Receptor Coactivator 2 in mouse liver

Project description:Steroid Receptor 2 (SRC-2) is a coactivator involved in hepatic metabolism. To better understand its temporal involvement in metabolism genome wide profiling was performed at 2 time ZT4 and ZT18 as well as in the SRC-2 knockout mouse.

2014-11-10 | GSE53039 | GEO

Genome-wide Profiling of SRC-2 (NCOA2) binding in mouse prostate [ChIP-Seq]

2014-06-30 | GSE54770 | GEO