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Genotoxicity of nitrosamines

ABSTRACT: We profiled the gene expression in eight chicken embryo livers in the 11th day of incubation. The eggs were injected on postincubation days 9, 10 and 11 with 4mg activation-dependent DNA reactive diethylnitrosamine (DEN) or its structural non-carcinogenic comparator, N-nitrosodiethanolamine (NDELA)DEN in 0.15 ml/egg deionized water. We found that both compounds induced simmilar massive transcriptomic alterations in the xenobioatic metabolism pathways when comparing with reference data in eggs injected with the vehicle only (deionized water).

ORGANISM(S): Gallus gallus

PROVIDER: GSE110906 | GEO | 2018/02/21

REPOSITORIES: GEO

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Gene expression in chicken embryo liver

Project description:We have profiled the gene expression in eight chicken embryo livers in the 11th day of incubation. Four eggs (labelled as WAT1-4) were injected with 0.15 ml/egg deionized water in days 9, 10 an 11 post incubation while four eggs (labeled CTR1-4) were kept as environmental controls. We found that water injections had a very small effect, regulating less than 1% of the genes. Both WAT and CTR data sets were used as references in further experiments for eggs expossed to various water-soluble carcinogenic compounds.

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