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AGO4 participates in germination during salinity

ABSTRACT: In this work we studied the participation of RdDM pathway in response to salinity during germination, we found that the RdDM promotes germination whule AGO4 has an additional RdDM-independent activity that negatively regulates the germination process.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE112051 | GEO | 2019/09/30

REPOSITORIES: GEO

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AGO4 participates in germination during salinity

Project description:In this work we studied the participation of RdDM pathway in response to salinity during germination, we found that the RdDM promotes germination while AGO4 null mutant negatively regulates the germination process.

2020-08-13 | GSE156093 | GEO

AGO4 participates in germination during salinity

Project description:AGO4 participates in germination during salinity

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AGO4 participates in germination during salinity

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Exogenous melatonin promotes rice seed germination under salinity through regulating reactive oxygen species and phytohormones

Project description:Melatonin plays a potential role in multiple plant developmental processes and stress response. However, there are no reports regarding exogenous melatonin promoting rice seed germination under salinity and nor about the underlying molecular mechanisms at genome-wide. Here, we revealed that exogenous application of melatonin conferred roles in promoting rice seed germination under salinity. The putative molecular mechanisms of exogenous melatonin in promoting rice seed germination under high salinity were further investigated through metabolomic and transcriptomic analyses. The results state clearly that the phytohormone contents were reprogrammed, the activities of SOD, CAT, POD were enhanced, and the total antioxidant capacity was activated under salinity by exogenous melatonin. Additionally, melatonin-pre-treated seeds exhibited higher concentrations of glycosides than non-treated seeds under salinity. Furthermore, exogenous melatonin alleviated the accumulation of fatty acids induced by salinity. Genome-wide transcriptomic profiling identified 7160 transcripts that were differentially expressed in NaCl, MT100 and control. Pathway and GO term enrichment analysis revealed that genes involved in the response to oxidative stress, hormone metabolism, heme building, mitochondrion, tricarboxylic acid transformation were altered after melatonin pre-treatment under salinity. This study provides the first evidence of the protective roles of exogenous melatonin in increasing rice seed germination under salt stress, mainly via activation of antioxidants and modulation of metabolic homeostasis.

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Redundant AGO-hook platforms and AGO4-DNA interaction in polV-directed RdDM

Project description:Small RNA-dependent pairing of AGO/PIWI-containing effector complexes to chromatin-bound nascent transcripts has been proposed as a universal mechanism for guiding RNA-mediated TGS in eukaryotes. Likewise, Pol V-dependent transcripts have been implicated in the targeting of AGO4 to chromatin in RNA-directed DNA methylation (RdDM) in plants. Here, we show that the AGO hook platforms of PolV and SPT5L, another component of the transcriptional complex, are functionally redundant yet essential for RdDM at a genome-wide level. Synthesis of Pol V transcripts is uncoupled from AGO4 recruitment in AGO hook-minus plants, challenging the prevailing RNA-based mechanism of AGO4 targeting to chromatin in RdDM. Transcription is essential to lock the PolV transcription complex into a stable and productive DNA-bound state potentiating interactions of AGO4 with DNA. Consistent with this idea, laser UV-assisted crosslinking shows specific AGO4-DNA interaction at RdDM loci, suggesting a revised model for Pol V-mediated DNA methylation in plants, which explains the exquisite specificity of methylation.

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AGO4 slicer activity reveals the passenger strand function of plant 23 nt siRNAs and the post-slicing retention of target RNAs mediating RNA-directed DNA methylation [WGBS]

Project description:In plants, transcriptional silencing by RNA-directed DNA methylation (RdDM) is mediated by ARGONAUTE 4 (AGO4) and 24 nt short-interfering RNAs (siRNAs) that are generated in parallel with 23 nt RNAs of unknown function. We show that 23 nt RNAs serve as the passenger strands of 23/24 nt duplexes loaded into AGO4. The 24 nt siRNAs then guide AGO4 slicing of the passenger strands, generating 11 and 12 nt cleavage products. Unexpectedly, we find that the 12 nt products remain associated with the guide strand-AGO4 complexes. Long noncoding RNAs generated at RdDM loci are similarly sliced and retained by AGO4. These results suggest a model in which RNA POLYMERASE V transcripts at target loci are sliced repeatedly as transcription elongation proceeds, sequentially releasing AGO4-siRNA-scaffold RNA complexes that independently recruit the RdDM machinery. Consistent with this hypothesis, plant lines expressing wild-type versus slicing-defective AGO4 show quantitative variation in cytosine methylation and siRNA levels within RdDM loci.

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AGO4 slicer activity reveals the passenger strand function of plant 23 nt siRNAs and the post-slicing retention of target RNAs mediating RNA-directed DNA methylation [smallRNA-seq]

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Project description:Using MethylC-Seq to provide single-base resolution of DNA methylation status in ago6-2, ago4-6 and ago4-6 ago6-2 double mutants MethylC-Seq: 3 mutants examined, ago6-2, ago4-6 single mutant and ago4-6 ago6-2 double mutants

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MethylC-Seq of ago6-2, ago4-6 and ago4-6 ago6-2 double mutants

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Expression data from 5-week-old Arabidopsis

Project description:AGO4 plays an important role in RNA-directed DNA methylation (RdDM). RdDM on specific genomic loci have the potential to silence the nearby protein coding gene. We used wild-type La-er and ago4-1 mutant Arabidopsis to identify AGO4-regulated genes. Five-weeks wild-type and ago4-1 mutant RNA were used for Affymetrix microarrays. Differentially expressed genes were identified to study the role of AGO4 in regulating plant development.

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