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AGO4 participates in germination during salinity

ABSTRACT: AGO4 participates in germination during salinity

PROVIDER: PRJNA656768 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12435869_1.fastq.gz	Fastqsanger.gz
	SRR12435869_2.fastq.gz	Fastqsanger.gz
	SRR12435870_1.fastq.gz	Fastqsanger.gz
	SRR12435870_2.fastq.gz	Fastqsanger.gz
	SRR12435871_1.fastq.gz	Fastqsanger.gz

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AGO4 participates in germination during salinity

Project description:AGO4 participates in germination during salinity

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AGO4 participates in germination during salinity

Project description:In this work we studied the participation of RdDM pathway in response to salinity during germination, we found that the RdDM promotes germination while AGO4 null mutant negatively regulates the germination process.

2020-08-13 | GSE156093 | GEO

AGO4 participates in germination during salinity

Project description:In this work we studied the participation of RdDM pathway in response to salinity during germination, we found that the RdDM promotes germination whule AGO4 has an additional RdDM-independent activity that negatively regulates the germination process.

2019-09-30 | GSE112051 | GEO

Exogenous melatonin promotes rice seed germination under salinity through regulating reactive oxygen species and phytohormones

Project description:Melatonin plays a potential role in multiple plant developmental processes and stress response. However, there are no reports regarding exogenous melatonin promoting rice seed germination under salinity and nor about the underlying molecular mechanisms at genome-wide. Here, we revealed that exogenous application of melatonin conferred roles in promoting rice seed germination under salinity. The putative molecular mechanisms of exogenous melatonin in promoting rice seed germination under high salinity were further investigated through metabolomic and transcriptomic analyses. The results state clearly that the phytohormone contents were reprogrammed, the activities of SOD, CAT, POD were enhanced, and the total antioxidant capacity was activated under salinity by exogenous melatonin. Additionally, melatonin-pre-treated seeds exhibited higher concentrations of glycosides than non-treated seeds under salinity. Furthermore, exogenous melatonin alleviated the accumulation of fatty acids induced by salinity. Genome-wide transcriptomic profiling identified 7160 transcripts that were differentially expressed in NaCl, MT100 and control. Pathway and GO term enrichment analysis revealed that genes involved in the response to oxidative stress, hormone metabolism, heme building, mitochondrion, tricarboxylic acid transformation were altered after melatonin pre-treatment under salinity. This study provides the first evidence of the protective roles of exogenous melatonin in increasing rice seed germination under salt stress, mainly via activation of antioxidants and modulation of metabolic homeostasis.

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Calmodulin-binding transcription activator (CAMTA) 6 is a key regulator of Na+ homeostasis and salinity stress responses during early germination

Project description:In view of the continuous salinization of arable lands world-wide, there is an urgent need to better understand the mechanisms underlying plant responses to salt stress at different stages of their development. We investigated the role of calmodulin (CaM)-binding transcription activator 6 (CAMTA6) under salinity stress during early germination in Arabidopsis. These analyses suggest that ABA signaling is involved in CAMTA6-dependent salt-responsive gene expression, consistent with the ABA hyper-tolerance phenotype and the lack of HKT1 response to ABA and NaCl in the camta6 mutants.

2019-06-16 | GSE116069 | GEO

proteome analysis of molecular mechanism in response to salt stress during seed germination in hulless barley

Project description:Seeds germination is seriously sensitive to salt stress. The mechanism in response to salt stress during seed germination is still little known. In this study, two genotypes of hulless barley lk621 and lk53 were selected to investigate the molecular mechanism of seeds salinity response during germination stage through RNA-seq and iTRAQ technologies

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Shotgun proteomic analysis of embryonic axis of soybean seeds during germination under salt stress

Project description:Salinity is one of the main environmental stresses worldwide limiting soybean growth and yield. Seed imbibition and radical emergence are generally less affected by salinity in soybean. Towards unraveling the mechanisms underlying salt tolerance in soybean at germination stage, a comprehensive quantitative proteomic analysis of proteins from soybean embryonic axis during germination sensu stricto (GSS) under saline conditions was performed. Application of 100 and 200 mmol L-1 NaCl at GSS was significantly accompanied by the change in abundance (>2-fold) of 97 and 75 proteins, respectively. Most of these proteins were involved in three major functions, namely stress response and defense, protein turnover and protection and primary metabolism. Our results pave the way towards the identification of suitable biomarkers useful for improving salt tolerance in soybean.

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2015-01-06 | E-GEOD-56388 | biostudies-arrayexpress

Shotgun proteomic analysis of embryonic axis of soybean seeds during germination under salt stress

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