Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Determine the genome-wide localization of nmad-1 and top-2 in the transgenic flag tagged strains

ABSTRACT: We report the chromatin immunoprecipitation followed by next generation sequencing (ChIP-seq) on flag tagged top-2 and nmad-1 transgeneic strains. We generated over 200 million reads from the IP'd DNA as well as input controls. We found that nmad-1 cobound with top-2 for approximately 80% of regions with p < 0.0001 (Fisher's exact test). Top-2 localization was also depedent on nmad-1 as half of the sites were no longer bound by top2 after nmad-1 deletion.

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE112487 | GEO | 2019/06/21

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Determine the genome-wide localization of nmad-1 and top-2 in the transgenic flag tagged strains

Project description:Determine the genome-wide localization of nmad-1 and top-2 in the transgenic flag tagged strains

| PRJNA448041 | ENA

Secondary phosphorylation in myocytes expressing FLAG-tagged and non-tagged phospho-mimetic cardiac troponin I.

Project description:Secondary phosphorylation develops in myocytes expressing phospho-mimetic cardiac troponin I (cTnI) but it is not known whether multiple substitutions (e.g. cTnISDTD and cTnIS4D) cause preferential phosphorylation of the remaining endogenous or the phospho-mimetic cTnI in intact myocytes. Western analysis was performed to determine whether the FLAG/total cTnI ratios are similar for phosphorylated versus total cTnI in myocytes expressing phospho-mimetic cTnI with Asp(D) substitutions at S43/45 plus S23/24 (cTnIS4D) or T144 (cTnISDTD). Representative Western analysis of phosphorylated S23/24 (p-S23/24) and S150 (p-S150) are presented along with re-probes using an antibody which detects all cTnI (MAB1691 Ab). The level of p-S150 also is compared to results obtained using single S43D and/or S45D phospho-mimetic substitutions. These results are discussed in more detail in Lang et al. [1].

| S-EPMC5651495 | biostudies-literature

Roof top Crust Metagenome

Project description:Roof top Crust Metagenome Metagenome

| PRJNA949969 | ENA

FLAG-tagged FOXM1A Plasmid

Project description:FLAG-tagged FOXM1A Plasmid

| PRJEB24606 | ENA

GCN2 suppresses TOP mRNA translation via LARP1

Project description:La-related protein 1 (LARP1) has been identified as a key translational inhibitor of terminal oligopyrimidine tract (TOP) mRNAs downstream of the nutrient sensing protein kinase complex, mTORC1. LARP1 exerts this inhibitory effect on TOP mRNA translation by binding to the mRNA cap and the adjacent 5’TOP motif, resulting in the displacement of the eIF4E complex from TOP mRNAs. In the present study, we identify a second nutrient sensing kinase GCN2 that converges on LARP1 to control TOP mRNA translation. GCN2 inhibits TOP mRNA translation via ATF4-dependent transcriptional induction of LARP1 mRNAs and GCN1-mediated recruitment of LARP1 to stalled ribosomes. We performed ATF4 ChIP-seq experiments in both WT and GCN2 KO MEFs with or without leucine deprivation.

2022-08-31 | GSE166590 | GEO

FLAG-tagged hnRNPM iCLIP in AGS cells

Project description:We performed hnRNPM iCLIP to identify the potential targets.

2021-06-16 | GSE178223 | GEO

Cucumis melo cultivar:Top Mark

Project description:Cucumis melo cultivar:Top Mark Raw sequence reads

| PRJNA358674 | ENA

Cucumis melo cultivar:Top Mark

Project description:Cucumis melo cultivar:Top Mark Raw sequence reads

| PRJNA358651 | ENA

Cucumis melo cultivar:Top Mark

Project description:Cucumis melo cultivar:Top Mark Raw sequence reads

| PRJNA358648 | ENA

Virus-like particles with FLAG-tagged envelope protein as a tetravalent dengue vaccine candidate.

Project description:The global incidence of dengue, which is caused by dengue virus (DENV) infection, has grown dramatically in recent decades and secondary infection with heterologous serotype of the virus may cause severe symptoms. Efficacious dengue vaccines should be able to provide long-lasting immunity against all four DENV serotypes simultaneously. In this study, we constructed a novel vaccine platform based on tetravalent dengue virus-like particles (DENV-LPs) in which envelope (E) protein carried a FLAG tag sequence at the position located not only in the exterior loop on the protruding domain but outside of dimerization interface of the protein. We demonstrated an effective strategy to produce the DENV-LPs by transient transfection with expression plasmids for pre-membrane and E proteins of DENV-1 to DENV-4 in mammalian cells and to concentrate and purify them with one-step affinity chromatography. Characteristic features of VLPs such as particle size, shape and density were comparable to flavivirus-like particles reported. The neutralizing activity against all four DENV serotypes was successfully induced by immunization with the purified tetravalent VLPs in mice. Simple, one-step purification systems for VLP vaccine platforms using epitope-tagging strategy should be advantageous for vaccine development not only for dengue but for emerging pandemics in the future.

| S-EPMC8413300 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data