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Npas4-regulated genes in mouse hippocampal neurons

ABSTRACT: we performed a DNA microarray experiment to identify activity-regulated genes that are misregulated in the absence of Npas4. Keywords: Primary neuron culture, depolarization, activity-dependent, Npas4

ORGANISM(S): Mus musculus

PROVIDER: GSE11258 | GEO | 2008/09/24

SECONDARY ACCESSION(S): PRJNA109195

REPOSITORIES: GEO

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RNAi profiling of mouse hippocampal neurons infected with lentivirus expressing Npas4-RNAi or control-RNAi to study Npas4-regulated genes

Project description:We performed a DNA microarray experiment to identify activity-regulated genes that are misregulated in the absence of Npas4. Experiment Overall Design: We infected mouse hippocampal neurons with lentivirus expressing Npas4-RNAi or control-RNAi @ 3 DIV and depolarized the neurons @ 8 DIV with 50 mM of KCl for 0, 1, 3 or 6 hours. Neurons were lysed, mRNA isolated and hybridized to Affymetrix arrays. Data were collected from 3 independent experiments.

2008-10-26 | E-GEOD-11258 | biostudies-arrayexpress

Genomic decoding of neuronal depolarization by stimulus-specific NPAS4 heterodimers, RNA sequencing

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An NPAS4:NuA4 complex couples synaptic activity to DNA repair

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