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Time course of gene expression of peritoneal macrophage from wild-type or LysM-St18flox mice after LPS stimulation

ABSTRACT: To assess the role of a transcription factor St18 in LPS response, peritoneal macrophage from wild-type or LysM-St18flox mice were stimulated with LPS and gene expressions were compared.

ORGANISM(S): Mus musculus

PROVIDER: GSE112683 | GEO | 2018/12/31

REPOSITORIES: GEO

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Gene expression of wild-type or LysM-St18flox M-CSF-derived macrophage or osteoclast

Project description:To assess the role of a transcription factor St18 in LPS response, M-CSF-derived macrophage (MDM) or osteoclast (OC) from wild-type or LysM-St18flox mice were prepared and gene expressions were compared.

2018-12-31 | GSE112684 | GEO

Expression data of LPS-stimulated macrophages in wild-type and LysM-Cre+;Akirin2fl/fl mice

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Time course of gene expression of peritoneal macrophage from wild-type or LysM-St18flox mice after LPS stimulation

Project description:Time course of gene expression of peritoneal macrophage from wild-type or LysM-St18flox mice after LPS stimulation

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Transcription profiling by array of LysMCre/Cre and KLF2?/? (LysMCre/Cre: KLF2 FL/FL) primary peritoneal macrophages treated with lipopolysaccharide (LPS) for 6 hours

Project description:Gene expression profile of LysMCre/Cre and KLF2?/? primary peritoneal macrophages following 6 hours of LPS treatment. We used microarrays to detail the global program of gene expression following LPS stimulation of LysMCre/Cre and KLF2?/? primary peritoneal macrophages. We identified distinct classes of genes that were altered following LPS stimulation. We injected 3 pairs of LysMCre/Cre (n=3) and KLF2?/? (n=3) mice with 2 ml of thioglycolate broth intraperitoneally. After 72 hours of thioglycolate injection, total cell suspension from peritoneal cavity were collected. These cells were seeded on 100mm tissue culture plates. Cells were allowed to attach for 4 hours and unattached cells were removed by washing with cell culture medium. These cells were placed in humidified incubator for additional 24 hours before LPS treatment. LPS was diluted to final concentration of 100ng/ml in culture medium and this medium was placed on peritoneal macrophages derived from LysM Cre/Cre and KLF2?/? mice for 6hours. Cells were lysed and total RNA was isolated and subjected to hybridization on Affymetrix microarrays.

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Differential gene expression analysis for BAL cells isolated from LysM Cre and Netrin1loxp/loxp LysM Cre mice after intratracheal LPS challenge

Project description:Purpose: The goal of this study is to compare NGS-derived transcriptome profiling (mRNA-seq) of expressed genes between LysM Cre and Netrin1loxp/loxp LysM Cre mice 3 days after intratracheal LPS challenge in order to explain the worsened outcomes and increased levels of inflammation we measure in the Netrin1loxp/loxp LysM Cre mice Methods: Three days after mice were treated with intratracheal injections of LPS, BAL cells were collected and then depleted for neutrophils using antibody-mediated depletion. The remaining cells were used for RNA isolation. mRNA profiles were generated by deep sequencing, in quadruplicate (one sample in the Netrin1loxp/loxp LysM Cre group was excluded as the sequence depth was only half compare to other 3 replicates), using paired-end 75-cycle sequencing on an Illumina NextSeq 550 System. Bases with quality scores < 20 and adapter sequences were removed from raw data with Cutadapt (v1.15), followed by alignment of clean RNA-seq reads to GRCm38 with STAR(v2.5.3a). Gene abundance was counted by HTseq-count uniquely-mapped reads number with default parameter using GencodeM15. Genes with > 5 reads in at least one sample were included for differential expression analysis by DESeq2 software.

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Compare RNA profile between LPS+CPN and LPS+CPN+GGOH treated peritoneal macrophages

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2023-07-20 | PXD041265 | Pride

Compare RNA profile between LPS+CPN and LPS+CPN+GGOH treated peritoneal macrophages

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High-throughput RNA sequencing analysis of mouse peritoneal cells from wild type and Lactoferrin knockout mouse during Lipopolysaccharide(LPS) stimulation

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