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Transcriptional Profiling of Host Cell Responses to Virulent Haemophilus parasuis

ABSTRACT: we investigated the host cell responses involved in the molecular pathways interaction in porcine aortic vascular endothelial cells (PAVECs) induced by H. parasuis

ORGANISM(S): Sus scrofa

PROVIDER: GSE113252 | GEO | 2018/04/18

REPOSITORIES: GEO

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Expression data from Porcine alveolar macrophages infection with Haemophilus parasuis (HPS)

2011-06-28 | GSE30172 | GEO

Expression data from Porcine alveolar macrophages of piglets experimentally infected with Haemophilus parasuis serotype 4 (HPS4) for 28 days

Project description:Porcine alveolar macrophages (PAMs) play impoartant role in innate immunity. Haemophilus parasuis is the etiological agent of Glasser’s disease in pigs. Haemophilus parasuis is the etiological agent of Glasser’s disease in pigs. We used microarrays to study the transcriptome of PAMs infection with HPS4.

2011-12-20 | GSE34544 | GEO

Expression data from Porcine alveolar macrophages infection with Haemophilus parasuis (HPS)

Project description:Porcine alveolar macrophages (PAMs) play impoartant role in innate immunity. Haemophilus parasuis is the etiological agent of Glasser’s disease in pigs. We used microarrays to study the transcriptome of PAMs infection with Haemophilus parasuis. Healthy Pigs were intratracheally challenged with H.parasuis strain 0165. And the PAMs were isolated at 6 dpi. RNA extraction were extracted from PAMs that obtained from infection pigs and control pigs and hybridization on Affymetrix microarrays. We sought to obtain the differently expressed genes that related to H.parasuis infection to understand the mechanism of PAMs against H.parasuis.

2011-06-28 | E-GEOD-30172 | biostudies-arrayexpress

Haemophilus parasuis gene expression the in lung

Project description:Purpose: Identify differentially expressed genes in pig lung, compared to growth on chocolate agar plate. Methods: In order to study the gene expression at this location, we sequenced ex vivo and in vivo H. parasuis transcriptome using a metatranscriptomic approach. Gene expression was compared with conventional plate culture. Results: down-regulation of anabolic and catabolic pathways, coupled with up-regulation of membrane-related genes involved in carbon acquisition, iron binding and pathogenesis. Conclusions: This data sheds some light on the scarcely studied in vivo transcriptome of H. parasuis, revealing nutritional virulence as an adaptive strategy for host survival.

2014-12-05 | GSE63851 | GEO

Transcriptional Profiling of Host Cell Responses to Virulent Haemophilus parasuis: New Insights into Pathogenesis.

Project description:Haemophilus parasuis is the causative agent of Glässer’s disease in pigs. H. parasuis can cause vascular damage, although the mechanism remains unclear. In this study, we investigated the host cell responses involved in the molecular pathway interactions in porcine aortic vascular endothelial cells (PAVECs) induced by H. parasuis using RNA-Seq. The transcriptome results showed that when PAVECs were infected with H. parasuis for 24 h, 281 differentially expressed genes (DEGs) were identified; of which, 236 were upregulated and 45 downregulated. The 281 DEGs were involved in 136 KEGG signaling pathways that were organismal systems, environmental information processing, metabolism, cellular processes, and genetic information processing. The main pathways were the Rap1, FoxO, and PI3K/Akt signaling pathways, and the overexpressed genes were determined and verified by quantitative reverse transcription polymerase chain reaction. In addition, 252 genes were clustered into biological processes, molecular processes, and cellular components. Our study provides new insights for understanding the interaction between bacterial and host cells, and analyzed, in detail, the possible mechanisms that lead to vascular damage induced by H. parasuis. This may lead to development of novel therapeutic targets to control H. parasuis infection.

| S-EPMC5983834 | biostudies-literature

Transcriptional Profiling of Host Cell Responses to Virulent Haemophilus parasuis

Project description:Transcriptional Profiling of Host Cell Responses to Virulent Haemophilus parasuis

| PRJNA450552 | ENA

Expression data from Porcine alveolar macrophages of piglets experimentally infected with Haemophilus parasuis serotype 4 (HPS4) for 28 days

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Global Gene Expression Profiles of Cecropin B Induces Resistance in Haemophilus parasuis

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Microarray analysis of Haemophilus parasuis SH0165 genomics induced by tilmicosin

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2012-11-10 | GSE34744 | GEO

Global Gene Expression Profiles of Cecropin B Induces Resistance in Haemophilus parasuis

Project description:To understand the mechanism of the adaptations, global gene expression profiles of the cecropin B--resistant strains of Haemophilus parasuis, The development of cecropin B (CB) resistance in H. parasuis SH0165 by exposing SH0165 using various concentrations of CB was investigated. One colony of bacterial cultures grown on TSA containing 30M-NM-<g/mL CB was named CBR30, CBR30 cultured in TSA without CB for 50 passages was named CBR30-50. we used microarray technology to analyze the variation of the H. parasuis SH0165 transcriptional profile in CBR30 and CBR30-50. Our analysis to identify several genes whose system could be involved in inorganic ion transport, amino acid transport, and metabolism. Quantitative PCR was used to validate the differential expression of selected genes. The H. parasuis SH0165 control group was normal culture with no tilmicosin at OD600 >0.3 (12 h). The H. parasuis CBR30 was normal culture with 30M-NM-<g/mL cecropin B at OD600 >0.3 (20 h). The H. parasuis CBR30-50 was normal culture with no cecropin B at OD600 >0.3 (12 h). Three independent experiments were performed on each group using a different sample for each experiment.

2012-12-11 | E-GEOD-42814 | biostudies-arrayexpress

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