Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from Porcine alveolar macrophages of piglets experimentally infected with Haemophilus parasuis serotype 4 (HPS4) for 28 days

ABSTRACT: Porcine alveolar macrophages (PAMs) play impoartant role in innate immunity. Haemophilus parasuis is the etiological agent of Glasser’s disease in pigs. Haemophilus parasuis is the etiological agent of Glasser’s disease in pigs. We used microarrays to study the transcriptome of PAMs infection with HPS4.

ORGANISM(S): Sus scrofa

PROVIDER: GSE34544 | GEO | 2011/12/20

SECONDARY ACCESSION(S): PRJNA151373

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Expression data from Porcine alveolar macrophages of piglets experimentally infected with Haemophilus parasuis serotype 4 (HPS4) for 28 days

Project description:Porcine alveolar macrophages (PAMs) play impoartant role in innate immunity. Haemophilus parasuis is the etiological agent of Glasser’s disease in pigs. Haemophilus parasuis is the etiological agent of Glasser’s disease in pigs. We used microarrays to study the transcriptome of PAMs infection with HPS4. Healthy Pigs were inoculated intranasally with 2 ml of 4.5×108CFU/ml colony forming units of HPS4 strain MD0322. And the PAMs were isolated at 28 dpi. RNA extraction were extracted from PAMs that obtained from infection pigs and control pigs and hybridization on Affymetrix microarrays.

2011-12-20 | E-GEOD-34544 | biostudies-arrayexpress

Expression data from Porcine alveolar macrophages infection with Haemophilus parasuis (HPS)

2011-06-28 | GSE30172 | GEO

Expression data from Porcine alveolar macrophages infection with Haemophilus parasuis (HPS)

Project description:Porcine alveolar macrophages (PAMs) play impoartant role in innate immunity. Haemophilus parasuis is the etiological agent of Glasser’s disease in pigs. We used microarrays to study the transcriptome of PAMs infection with Haemophilus parasuis. Healthy Pigs were intratracheally challenged with H.parasuis strain 0165. And the PAMs were isolated at 6 dpi. RNA extraction were extracted from PAMs that obtained from infection pigs and control pigs and hybridization on Affymetrix microarrays. We sought to obtain the differently expressed genes that related to H.parasuis infection to understand the mechanism of PAMs against H.parasuis.

2011-06-28 | E-GEOD-30172 | biostudies-arrayexpress

Global Gene Expression Profiles of Cecropin B Induces Resistance in Haemophilus parasuis

2012-12-11 | GSE42814 | GEO

Haemophilus parasuis

Project description:Haemophilus parasuis Raw sequence reads

| PRJNA980943 | ENA

Global Gene Expression Profiles of Cecropin B Induces Resistance in Haemophilus parasuis

Project description:To understand the mechanism of the adaptations, global gene expression profiles of the cecropin B--resistant strains of Haemophilus parasuis, The development of cecropin B (CB) resistance in H. parasuis SH0165 by exposing SH0165 using various concentrations of CB was investigated. One colony of bacterial cultures grown on TSA containing 30M-NM-<g/mL CB was named CBR30, CBR30 cultured in TSA without CB for 50 passages was named CBR30-50. we used microarray technology to analyze the variation of the H. parasuis SH0165 transcriptional profile in CBR30 and CBR30-50. Our analysis to identify several genes whose system could be involved in inorganic ion transport, amino acid transport, and metabolism. Quantitative PCR was used to validate the differential expression of selected genes. The H. parasuis SH0165 control group was normal culture with no tilmicosin at OD600 >0.3 (12 h). The H. parasuis CBR30 was normal culture with 30M-NM-<g/mL cecropin B at OD600 >0.3 (20 h). The H. parasuis CBR30-50 was normal culture with no cecropin B at OD600 >0.3 (12 h). Three independent experiments were performed on each group using a different sample for each experiment.

2012-12-11 | E-GEOD-42814 | biostudies-arrayexpress

Expression data from Porcine alveolar macrophages of piglets experimentally infected with Streptococcus suis serotype 2 (SS2) for 7 days

Project description:Porcine alveolar macrophages (PAMs) play an important role in innate immunity. Streptococcus suis serotype 2 is a pathogen responsible for several diseases in both pigs and humans. We used microarrays to study the transcriptome of PAMs infected with SS2. Healthy pigs were inoculated intranasally with 2 ml of 4.84×10^6 colony-forming units of SS2 strain SC19. The PAMs were isolated at 7 dpi. RNA was extracted from PAMs obtained from infected pigs and control pigs, and hybridized on Affymetrix microarrays.

2011-07-14 | E-GEOD-30696 | biostudies-arrayexpress

Haemophilus parasuis genome comparisons

Project description:Haemophilus parasuis genome comparisons

| PRJEB2342 | ENA

Haemophilus parasuis gene expression the in lung

Project description:Purpose: Identify differentially expressed genes in pig lung, compared to growth on chocolate agar plate. Methods: In order to study the gene expression at this location, we sequenced ex vivo and in vivo H. parasuis transcriptome using a metatranscriptomic approach. Gene expression was compared with conventional plate culture. Results: down-regulation of anabolic and catabolic pathways, coupled with up-regulation of membrane-related genes involved in carbon acquisition, iron binding and pathogenesis. Conclusions: This data sheds some light on the scarcely studied in vivo transcriptome of H. parasuis, revealing nutritional virulence as an adaptive strategy for host survival.

2014-12-05 | GSE63851 | GEO

Expression data from Porcine alveolar macrophages of piglets experimentally infected with Streptococcus suis serotype 2 (SS2) for 7 days

2011-07-15 | GSE30696 | GEO