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RNA-seq of 10dpp wild type and Dazl SKO mouse testes

ABSTRACT: The autosomal gene Dazl is a member of highly conservative DAZ family, all of which contains a RNA binding domain and expresses in germ cells. In mice, Dazl plays an essential role in germ cell development, germ cell lost in Dazl knockout mouse on pure C57BL/6 strain background. Here we generated conditional DAZL knockout mice which allowed us to examine the roles of DAZL in postnatal spermatogenesis without affecting its early function in PGC. RNA was extracted from 10dpp wild type and Stra8-cre KO mouse testes to perform RNA-seq, StringTie software was used to assess the expression level by formula log2(FPKM+1).

ORGANISM(S): Mus musculus

PROVIDER: GSE114042 | GEO | 2019/10/09

REPOSITORIES: GEO

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Dazl HITS-CLIP on 25dpp C57BL/6 mouse testes

Project description:The autosomal gene Dazl is a member of highly conservative DAZ family, all of which contains a RNA binding domain and expresses in germ cells. In mice, Dazl plays an essential role in germ cell development, germ cell lost in Dazl knockout mouse on pure C57BL/6 strain background. Here we use HITS-CLIP to globally characterize the genome-wide target RNA of Dazl in mouse testes. Sequencing data provides large quantities of clues to help us to get close to the truth of Dazl function. We find over 1700 RNAs are bind by Dazl mainly at 3’UTR and the genes associated with transcription, RNA splicing and reproductive cellular process are enriched significantly.

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RNA-seq of 10dpp wild type and Dazl SKO mouse testes

Project description:RNA-seq of 10dpp wild type and Dazl SKO mouse testes

| PRJNA462763 | ENA

Mus musculus Dazl-deficient fetal ovary transcriptome

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An RNA-interacting protein network regulates pluripotency in mammalian primordial germ cells (Dazl knockdown)

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