Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Integrin alpha6-beta4 control the expression of genes associated with cell motility, invasion and metastasis

ABSTRACT: The objective of this study was to determine the gene expression changes mediated by the alpha6beta4 integrin using MDA-MB-435 breast carcinoma cell line under normal culturing conditions (10% FCS in DMEM). Keywords: Transfection (stable)

ORGANISM(S): Homo sapiens

PROVIDER: GSE11466 | GEO | 2008/12/01

SECONDARY ACCESSION(S): PRJNA106385

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcription profiling of human MDA-MB-435 breast carcinoma cell line transfected with the integrin beta4 subunit revealsIntegrin alpha6-beta4 control the expression of genes associated with cell motility, invasion and metastasis

Project description:The objective of this study was to determine the gene expression changes mediated by the alpha6beta4 integrin using MDA-MB-435 breast carcinoma cell line under normal culturing conditions (10% FCS in DMEM). Experiment Overall Design: Comparison of clones of MDA-MB-435 cells transfected with the integrin beta4 subunit (which results in cell surface expression of the integrin apha6beta4 integrin; clones 3A7 and 5B3) to those transfected with vector (mock; 6D2 and 6D7) only.

2009-01-13 | E-GEOD-11466 | biostudies-arrayexpress

Micro-RNAs expressed in oligometastases inhibit genes involved with adhesion, invasion and motility

Project description:Analysis of MDA-MB-231 cells 48 hours after transfection with non-targeting control, micro-RNA mimics or corresponding micro-RNA inhibitor. The hypothesis tested was that micro-RNAs previously identified as expressed in oligometastatic patients' metastases at a higher level than polymetastatic patients' metastases suppress genes regulating adhesion, motility, invasion and growth. Results showed that multiple micro-RNAs can suppress multiple genes involved in these pathways. Total RNA was obtained from MDA-MB-231 cells after transfection with micro-RNA mimic, micro-RNA inhibitor, or non-targeting control MB231, performed in duplicate

2015-03-01 | E-GEOD-60766 | biostudies-arrayexpress

Micro-RNAs expressed in oligometastases inhibit genes involved with adhesion, invasion and motility

2015-03-01 | GSE60766 | GEO

Nm23-H1 suppresses tumor cell motility by downregulating the LPA receptor

Project description:Exogenous overexpression of the metastasis suppressor gene Nm23-H1 reduces the metastatic potential of multiple types of cancer cells. In addition, Nm23-H1 expression suppresses in vitro tumor cell motility and invasion, important correlates of metastasis. The molecular mechanism by which Nm23-H1 suppresses metastasis, motility and invasion has yet to be defined. However, mutational analysis of Nm23-H1 has revealed that substitution mutant P96S and S120G are unable to inhibit cell motility. To elucidate the molecular mechanism of Nm23-H1 motility suppression, we performed expression microarray analysis of an MDA-MB- 435 carcinoma cell line overexpressing wild-type Nm23-H1 and cross-compared the expression profile to lines expressing P96S and S120G mutants. Nine genes, MET, PTN, SMO, FZD1, L1CAM, MMP2, NETO2, CTGF and EDG2, were down regulated by wild-type but not by mutant Nm23-H1 expression (p<0.05). Reduced expression of these genes coincident with elevated Nm23-H1 expression was observed in human breast tumor cohorts, a panel of breast carcinoma cell lines and hepatocellular carcinomas from control and Nm23-M1 knockout mice. The functional significance of down regulated genes was assessed by transfection and in vitro motility assays. Only EDG2 overexpression significantly restored motility to Nm23-H1- suppressed cancer cells and it enhanced motility by 60-fold in these cells. In addition, silencing EDG2 expression with siRNA, reduced the motile phenotype of metastatic breast cancer cells. These data suggest that Nm23-H1 suppresses metastasis, at least in part, through downregulation of EDG2 expression and that compounds directed toward EDG2 may be useful for treating aggressive, low Nm23-H1-expressing breast tumors. Keywords: Human MDA-MB-435 Breast cancer derived cell lines

2007-04-30 | GSE7549 | GEO

Integrin α3b1-dependent gene expression in MDA-MB-231 breast cancer cells

Project description:Gene-level and exon-level analysis of gene expression in MDA-MB-231 cells that stably express control shRNA or integrin α3-targeting shRNA. The laminin-332-binding integrin α3b1 is expressed highly in many breast cancer cells, but its roles in regulating gene expression programs that promote breast cancer progression have not been explored. In order to identify genes that are regulated by α3b1 in human breast cancer cells, we used a lentiviral approach to express an α3-targeting shRNA to suppress integrin α3b1 in MDA-MB-231 cells, and we identified subsequent changes in gene expression and alternate exon useage. We used the Affymetrix Human Exon 1.0 ST platform to analyze biological replicates of MDA-MB-231 cells that were transduced with lentivirus to stably express either control shRNA or α3-targeting shRNA. Array data was processed by Affymetrix Exon Array Computational Tool.

2014-05-02 | E-GEOD-43097 | biostudies-arrayexpress

Integrin α3b1-dependent gene expression in MDA-MB-231 breast cancer cells

2014-05-02 | GSE43097 | GEO

Transcriptional profile of bone marrow-derived macrophages from Cd28 KO mice

Project description:Analysis of the transcriptome of bone marrow-derived macrophages from Cd28 KO mice. Methods: C57BL/6J (WT) and Cd28 KO (B6.129S2-Cd28tm1Mak/J) mice were used. Bone marrow-derived macrophages were obtained by flushing the femurs of 6- to 10-wk-old C57BL/6, and Cd28 KO mice, and culturing cells during 7 days in DMEM supplemented with 10% FCS and 50 mM 2-ME, containing human M-CSF (25 ng/ml), with cytokine addition every 2 days.

2022-12-01 | GSE157837 | GEO

Tinagl1 Suppresses Triple-negative Breast Cancer Progression by Simultaneously Targeting Integrin/FAK and EGFR Signaling Pathways

Project description:Enforced Tinagl1 expression in lung metastatic MDA-MB231 subline LM2 cells suppresses lung metastasis by inhibiting EGFR and Integrin/FAK activation

2018-12-31 | GSE99507 | GEO

The melanoma cell line MDA-MB-435 adhesome in long-term culture and its comparison to the adhesome upon integrin αV knockdown

Project description:Using mass spectrometry–based proteomics, we analysed the components of integrin adhesion complexes of MDA-MB-435S cells and two MDA-MB-435S-derived integrin αV-specific shRNA-expressing cell clones. Among integrins, we detected αV and β5 integrin subunits in the MDA-MB-435S adhesome, thus showing that in long term culture these cells use preferentially integrin αVβ5 for adhesion. Our data represents the first published adhesome of αVβ5 integrin heterodimer.

2020-02-17 | PXD016837 | Pride

Effect of Exportin-5 expression on Mouse fibroblasts in cell cycle re-entry phase

Project description:To analyze the effect of Exportin-5 expression on the MEF cells in cell cycle re-entry phase, we have employed whole genome microarray expression profiling on the MEF cells in cell cycle re-entry phase with and without down regulation of Exportin-5 gene. Mouse MEF cells were transfected with 60nM of siRNA targeting either Exportin-5 or negative control, and incubated for 12 hours. After incubation, cells were starved with DMEM containing 0.2% FCS for 48 hours and re-fed with DMEM containing 20%FCS for 24 hours, along with second siRNA transfection. Two independant expreiments were preformed.

2013-01-01 | E-GEOD-25498 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data