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Ret and substrate-derived TGFb maverick regulate space-filling dendrite growth in Drosophila sensory neurons

ABSTRACT: Microarray profiling of amplified total RNA isolated from FACS-sorted class IV da neurons

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE115500 | GEO | 2018/08/17

REPOSITORIES: GEO

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Ret and Substrate-Derived TGF-? Maverick Regulate Space-Filling Dendrite Growth in Drosophila Sensory Neurons.

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Ret and substrate-derived TGFb maverick regulate space-filling dendrite growth in Drosophila sensory neurons

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The ELAV/Hu protein Found in neurons regulates cytoskeletal and ECM adhesion inputs for space-filling dendrite growth

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Project description:Microarray profiling of amplified total RNA isolated from FACS-sorted class IV da neurons or body wall filets.

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Project description:Microarray profiling of amplified total RNA isolated from FACS-sorted class IV da neurons or body wall filets. 3 to 5 independent replicates collected from each sample group, RNA isolated and amplified. Hybridized as a one color experiment.

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Project description:Precise patterning of dendritic arbors is critical for the wiring and function of neural circuits. Dendrite-extracellular matrix (ECM) adhesion ensures that the dendrites of Drosophila dendritic arborization (da) sensory neurons are properly restricted in a 2D space, and thereby facilitates contact-mediated dendritic self-avoidance and tiling. However, the mechanisms regulating dendrite-ECM adhesion in vivo are poorly understood. Here, we show that mutations in the semaphorin ligand sema-2b lead to a dramatic increase in self-crossing of dendrites due to defects in dendrite-ECM adhesion, resulting in a failure to confine dendrites to a 2D plane. Furthermore, we find that Sema-2b is secreted from the epidermis and signals through the Plexin B receptor in neighboring neurons. Importantly, we find that Sema-2b/PlexB genetically and physically interacts with TORC2 complex, Tricornered (Trc) kinase, and integrins. These results reveal a novel role for semaphorins in dendrite patterning and illustrate how epidermal-derived cues regulate neural circuit assembly.

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Project description:Class I and IV Drosophila dendritic arborization sensory neurons were isolated via magnetic bead sorting and total RNA isolated from the samples was used for gene expression profiling. Elucidating the molecular mechanisms controlling dendrite development is key to understanding the pivotal role these structures play in influencing synaptic integration and neural function. Despite significant advances in this field, genetic pleiotropy remains a significant impediment to investigating such complex developmental processes. To circumvent this problem, we have applied class specific neuron transcriptional expression profiling coupled to an in vivo RNAi functional validation screen in order to dissect the molecular bases of Drosophila class I and class IV dendritic arborization (da) neuron dendritogenesis. Gene expression profiling of class I and IV da neurons, isolated via magnetic bead sorting (using protocols as described in Iyer et al., 2009), was performed at the third instar larval stage of development from independent cell isolations from 40-50 age-matched third instar larvae expressing mCD8::GFP under the control of either GAL4ppk.1.9 driver or Gal80ppk; GAL4221. For controls, age matched whole larval homogenate RNA was used.

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