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Coupled single-cell epigenome editing and profiling reveals causal gene regulatory networks [Bulk ATAC-seq]

ABSTRACT: Here we present Perturb-ATAC, a method which combines multiplexed CRISPR interference or knockout with genome-wide chromatin accessibility profiling in single cells, based on the simultaneous detection of CRISPR guide RNAs and open chromatin sites by Assay of Transposase-accessible Chromatin with sequencing (ATAC-seq). We applied Perturb-ATAC to transcription factors (TFs), chromatin-modifying factors, and noncoding RNAs (ncRNAs) in ~3,700 single cells, encompassing more than 75 unique genotype-phenotype relationships.

ORGANISM(S): Homo sapiens

PROVIDER: GSE116247 | GEO | 2018/12/20

REPOSITORIES: GEO

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Coupled single-cell epigenome editing and profiling reveals causal gene regulatory networks [scATAC-seq]

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