The Arabis alpina APETALA2 orthologue delays flowering by repressing floral meristem identity genes during vernalization
Ontology highlight
ABSTRACT:
ORGANISM(S): Arabis alpina
PROVIDER: GSE117977 | GEO | 2019/02/27
REPOSITORIES: GEO
Similar Datasets
Project description:The Arabis alpina APETALA2 orthologue delays flowering by repressing floral meristem identity genes during vernalization
| PRJNA483886 | ENA
Project description:Divergence of regulatory networks governed by the orthologous transcription factors FLC and PEP1 in Brassicaceae species
| PRJNA352782 | ENA
Project description:The floral repressor APETALA2 (AP2) in Arabidopsis regulates flowering through the age pathway. The AP2 ortholog in the alpine perennial Arabis alpina, PERPETUAL FLOWERING 2 (PEP2), was previously reported to control flowering through the vernalization pathway via enhancing the expression of another floral repressor PERPETUAL FLOWERING 1 (PEP1), the ortholog of Arabidopsis FLOWERING LOCUS C (FLC). However, PEP2 also regulates flowering independently of PEP1. To characterize the function of PEP2, we analyzed the transcriptomes of pep2 and pep1 mutants. The majority of differentially expressed genes were detected between pep2 and the wild type or between pep2 and pep1, highlighting the importance of the PEP2 role that is independent of PEP1. Here, we demonstrate that PEP2 activity prevents the up-regulation of the A. alpina floral meristem identity genes FRUITFUL (AaFUL), LEAFY (AaLFY), and APETALA1 (AaAP1), ensuring floral commitment during vernalization. Young pep2 seedlings respond to vernalization, suggesting that PEP2 regulates the age-dependent response to vernalization independently of PEP1. The major role of PEP2 through the PEP1-dependent pathway takes place after vernalization, when it facilitates PEP1 activation both in the main shoot apex and in axillary branches. These multiple roles of PEP2 in the vernalization response contribute to the A. alpina life cycle.
| S-EPMC6363098 | biostudies-literature
Project description:Arabis alpina, similar to woody perennials, has a complex architecture with a zone of axillary vegetative branches and a zone of dormant buds that serve as perennating organs. We show that floral development during vernalization is the key for shaping the dormant bud zone by facilitating a synchronized and rapid growth after vernalization and thereby causing an increase in auxin transport, response and endogenous indole-3-acetic acid (IAA) levels in the stem. Floral development during vernalization is associated with the development of axillary buds in subapical nodes. Our transcriptome analysis indicated that these buds are not dormant during vernalization but only reach sustained growth after the return to warm temperatures. Floral and subapical vegetative branches grow after vernalization and inhibit the development of the buds below. Dormancy in these buds is regulated across the A. alpina life cycle by low temperatures and by apical dominance in a BRANCHED 1 dependent mechanism.
2020-03-01 | GSE126944 | GEO
Project description:Transcriptomic profile of Arabis alpina apex enriched material. We compared apices from plants grown for 2 weeks in long days (20M-BM-0C) (juvenile) with apices from plants grown for 8 weeks in long days (20M-BM-0C) (adult) and apices from plants grown for 2 weeks and then shifted in vernalization for 4 weeks with apices from plants grown for 8 weeks and than shifted to vernalization for 4 weeks. 2x2 factorial design. 2 starting ages (2 weeks and 8 weeks) and 2 developmental conditions (before and after vernalization). 4 biological replicates for each condition (16 arrays). Reference: pool of the 16 arrays.
2013-05-27 | E-GEOD-40455 | biostudies-arrayexpress
Project description:Transcriptomic profile of Arabis alpina apex enriched material. We compared apices from plants grown for 2 weeks in long days (20°C) (juvenile) with apices from plants grown for 8 weeks in long days (20°C) (adult) and apices from plants grown for 2 weeks and then shifted in vernalization for 4 weeks with apices from plants grown for 8 weeks and than shifted to vernalization for 4 weeks.
2013-05-27 | GSE40455 | GEO
Project description:BACKGROUND: In Arabidopsis thaliana (A. thaliana) the WD40 protein TRANSPARENT TESTA GLABRA1 (TTG1) controls five traits relevant for the adaptation of plants to environmental changes including the production of proanthocyanidin, anthocyanidin, seed coat mucilage, trichomes and root hairs. The analysis of different Brassicaceae species suggests that the function of TTG1 is conserved within the family. RESULTS: In this work, we studied the function of TTG1 in Arabis alpina (A. alpina). A comparison of wild type and two Aattg1 alleles revealed that AaTTG1 is involved in the regulation of all five traits. A detailed analysis of the five traits showed striking phenotypic differences between A. alpina and A. thaliana such that trichome formation occurs also at later stages of leaf development and that root hairs form at non-root hair positions. CONCLUSIONS: The evolutionary conservation of the regulation of the five traits by TTG1 on the one hand and the striking phenotypic differences make A. alpina a very interesting genetic model system to study the evolution of TTG1-dependent gene regulatory networks at a functional level.
| S-EPMC3904473 | biostudies-literature
Project description:Polycarpic perennials maintain vegetative growth after flowering. PERPETUAL FLOWERING 1 (PEP1), the orthologue of FLOWERING LOCUS C (FLC) inArabis alpina regulates flowering and contributes to polycarpy in a vernalisation-dependent pathway. pep1 mutants do not require vernalisation to flower and have reduced return to vegetative growth as all of their axillary branches become reproductive. To identify additional genes that regulate flowering and contribute to perennial traits we performed an enhancer screen of pep1. Using mapping-by-sequencing, we cloned a mutant (enhancer of pep1-055, eop055), performed transcriptome analysis and physiologically characterised the role it plays on perennial traits in an introgression line carrying the eop055 mutation and a functional PEP1 wild-type allele. eop055 flowers earlier than pep1 and carries a lesion in the A. alpina orthologue of the APETALA2 (AP2)-like gene, TARGET OF EAT2 (AaTOE2). AaTOE2 is a floral repressor and acts upstream of SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE 5 (AaSPL5). In the wild-type background, which requires cold treatment to flower, AaTOE2 regulates the age-dependent response to vernalisation. In addition, AaTOE2 ensures the maintenance of vegetative growth by delaying axillary meristem initiation and repressing flowering of axillary buds before and during cold exposure. We conclude that AaTOE2 is instrumental in fine tuning different developmental traits in the perennial life cycle of A. alpina.
2021-01-26 | GSE145718 | GEO
Project description:Mechanisms of age-dependent response to winter temperature in perennial flowering of Arabis alpina.
| PRJNA174078 | ENA
Project description:Whole-genome resequencing of Arabis alpina populations from Swiss Alps.
| PRJNA489364 | ENA