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RocA has serotype-specific gene regulatory and pathogenesis activity in serotype M28 group A streptococcus

ABSTRACT: RNA-seq experiments of a wild-type and isogenic rocA deletion mutant serotype M28 group A streptococcus strain at two time points

ORGANISM(S): Streptococcus pyogenes

PROVIDER: GSE118609 | GEO | 2018/08/27

REPOSITORIES: GEO

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Polymorphisms in RocA contribute to the molecular pathogenesis of serotype M28 group A streptococcus

Project description:RNA-seq experiments of serotype M28 group A streptococcus strains with mutations in rocA at two time points

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Regulatory Rewiring Confers Serotype-Specific Hyper-Virulence in the Human Pathogen Group A Streptococcus

Project description:We sequenced mRNA transcripts from three isogenic M3 serotype GAS strains, parental (MGAS10870), complement (10870::rocAM1), and deletion (10870ΔrocA). There were no significant changes between the parental and revertant strain. Comparison of the parental and complemented strain revealed several virulence factors were up-regulated in the parental strain where RocA function was diminished. We concluded that RocA, through direct or indirect mechanisms, is able to control numerous virulence genes and this lack of RocA regulation increases expression of virulence factors, which contributes to the hyper-virulent state of serotype M3 GAS.

2015-08-24 | GSE68277 | GEO

Regulatory Rewiring Confers Serotype-Specific Hyper-Virulence in the Human Pathogen Group A Streptococcus

Project description:We sequenced mRNA transcripts from three isogenic M3 serotype GAS strains, parental (MGAS10870), complement (10870::rocAM1), and deletion (10870?rocA). There were no significant changes between the parental and revertant strain. Comparison of the parental and complemented strain revealed several virulence factors were up-regulated in the parental strain where RocA function was diminished. We concluded that RocA, through direct or indirect mechanisms, is able to control numerous virulence genes and this lack of RocA regulation increases expression of virulence factors, which contributes to the hyper-virulent state of serotype M3 GAS. GAS strains were grown to mid-exponential phase, total RNA isolated, rRNA depleted, cDNA libraries synthesized, and libraries analyzed using Illumina MiSeq and CLC Genomics Workbench version 7.5.1 RNA-seq software.

2015-08-24 | E-GEOD-68277 | biostudies-arrayexpress

Stationary phase gene regulation in M1 GAS by the group A Streptococcus protein RocA

Project description:We used RNA-Seq to assess how RocA regulates gene expression in stationary phase cultures of serotype M1 GAS

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Investigation into whether the RD2 element in serotype M28 isolates of group A Streptococcus influences global gene expression

Project description:We used RNA-Seq to compare a parental M28 isolate with an isogenic mutant in which the RD2 element had been deleted.

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RocA Has Serotype-Specific Gene Regulatory and Pathogenesis Activities in Serotype M28 Group A Streptococcus.

Project description:Serotype M28 group A streptococcus (GAS) is a common cause of infections such as pharyngitis ("strep throat") and necrotizing fasciitis ("flesh-eating" disease). Relatively little is known about the molecular mechanisms underpinning M28 GAS pathogenesis. Whole-genome sequencing studies of M28 GAS strains recovered from patients with invasive infections found an unexpectedly high number of missense (amino acid-changing) and nonsense (protein-truncating) polymorphisms in rocA (regulator of Cov), leading us to hypothesize that altered RocA activity contributes to M28 GAS molecular pathogenesis. To test this hypothesis, an isogenic rocA deletion mutant strain was created. Transcriptome sequencing (RNA-seq) analysis revealed that RocA inactivation significantly alters the level of transcripts for 427 and 323 genes at mid-exponential and early stationary growth phases, respectively, including genes for 41 transcription regulators and 21 virulence factors. In contrast, RocA transcriptomes from other GAS M protein serotypes are much smaller and include fewer transcription regulators. The rocA mutant strain had significantly increased secreted activity of multiple virulence factors and grew to significantly higher colony counts under acid stress in vitro RocA inactivation also significantly increased GAS virulence in a mouse model of necrotizing myositis. Our results demonstrate that RocA is an important regulator of transcription regulators and virulence factors in M28 GAS and raise the possibility that naturally occurring polymorphisms in rocA in some fashion contribute to human invasive infections caused by M28 GAS strains.

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Stationary phase gene regulation by the group A Streptococcus protein RocA

Project description:We used RNA-Seq to assess how RocA regulates gene expression in stationary phase GAS cultures

2019-11-22 | GSE131239 | GEO

RocA regulates gene expression in serotype M1 group A Strep (GAS, aka S. pyogenes)

Project description:We used RNA-Seq to assess whether the rocA gene regulates gene expression in M1 GAS

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Streptococcus pyogenes

Project description:RocA is an important regulator with serotype-specific pathogenesis activity in serotype M28 group A Streptococcus

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Evaluation of ccpA deletion on global gene expression of three M serotype strains of group A streptococcus

Project description:We sought to determine how deletion of ccpA affects the gene expression profile in three M serotype strains of group A streptococcus

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