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Whole transcriptome analysis in 661W photoreceptor cells

ABSTRACT: This experiment aimed to characterise the complete transcriptional profile of 661W cells to identify their origin as cone photoreceptor cells. Cells were either grown in complete media or subjected to 72 hours of serum starvation to encourage differentiation.

ORGANISM(S): Mus musculus

PROVIDER: GSE119190 | GEO | 2018/08/30

REPOSITORIES: GEO

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The deubiquitinase Otud7b suppresses cone photoreceptor degeneration in mouse models of retinal degenerative diseases

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Whole transcriptome analysis in 661W photoreceptor cells

Project description:Whole transcriptome analysis in 661W photoreceptor cells

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Mouse retina single-cell RNA-seq of WT and two Nr2e3 mutant models to explore the role of Nr2e3 in cone and rod photoreceptor differentiation

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Retinoic acid signaling targets within the embryonic zebrafish eye during photoreceptor differentiation

Project description:The signaling molecule retinoic acid (RA) regulates rod and cone photoreceptor fate, differentiation, and survival. The purpose of this study was to identify eye-specific genes controlled by RA during photoreceptor differentiation in the zebrafish. 6 samples; 3 replicates of 2 conditions

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Project description:Vertebrate vision is mediated by two kinds of photoreceptors, rods and cones, responsible for dim- and bright-light vision, respectively. Gene expression differences among cone subtypes remain poorly understood compared with rods. We generated single-cell transcriptome data using a droplet-based approach to reveal the extent of gene expression diversity among adult zebrafish photoreceptor subtypes. Populations of photoreceptor cells were enriched by using the transgenic zebrafish lines, Tg(rho:EGFP)ja2Tg and Tg(gnat2:EGFP)ja23Tg, which express GFP in rods and all cone subtypes, respectively. By analyzing the single-cell transcriptomes, we found that in addition to the four canonical zebrafish cone types (ultraviolet, blue, green and red), there exist subpopulations of green and red cones in the ventral retina that express red-shifted opsin paralogs (opn1mw4 and opn1lw1). This work lays a foundation for future studies aimed at understanding how molecular differences among cone subtypes affect photoreceptor function.

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Retinoic acid signaling targets within the embryonic zebrafish eye during photoreceptor differentiation

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Investigating cone photoreceptor development using patient-derived NRLnull retinal organoids

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