Project description:Analysis of change in transcriptosome after TRα or TRβ knockdown in hADSC. Analysis of effects of siRNA transfection (siCtrl, siTRα, siTRβ or siETV5) in hADSC at gene expression level.The hypothesis tested in the present study was that unliganded TRα and TRβ have distinctive roles in these cells. Total RNA was obtained from hADSC, transfected with siCtrl, siTRα, siTRβ or ETV5. siTRα, siTRβ or siETV5 samples were compared to siCtrl samples.
Project description:Analysis of change in transcriptional networking and disection of the TRalpha and TRbeta roles in hADSC, and during differentiation of hADSC towadrd different lineages. Total RNA was obtained from hADSC, and adipocytes, chondrocytes and osteoblasts differentiated from hADSC treated with triiodothytonine (T3) for 0 and 18 h. T3 response in each cell line was analyzed.
Project description:Analysis of change in transcriptosome after TRα or TRβ knockdown in hADSC. Analysis of effects of siRNA transfection (siCtrl, siTRα, siTRβ or siETV5) in hADSC at gene expression level.The hypothesis tested in the present study was that unliganded TRα and TRβ have distinctive roles in these cells.
Project description:Transcriptional abundance in each of senescent populations from three cell lines (WS1, WI38 and BJ) is compared with abundance in isogenic early passage proliferating cells and also with abundance in early passage quiescent cells. A strain or line experiment design type assays differences between multiple strains, cultivars, serovars, isolates, lines from organisms of a single species. Keywords: strain_or_line_design
Project description:Analysis of change in transcriptosome after KLF2 and KLF8 knockdown in hADSC. Analysis of effects of siRNA transfection (siCtrl, siKLF2 or siKLF8) in hADSC at gene expression level.The hypothesis tested in the present study was that different KLF members have distinctive roles in these cells.
Project description:Transcriptional abundance in each of senescent populations from three cell lines (WS1, WI38 and BJ) is compared with abundance in isogenic early passage proliferating cells and also with abundance in early passage quiescent cells. A strain or line experiment design type assays differences between multiple strains, cultivars, serovars, isolates, lines from organisms of a single species. Computed
Project description:Analysis of change in transcriptional networking and disection of the TRalpha and TRbeta roles in hADSC, and during differentiation of hADSC towadrd different lineages.
Project description:Transcriptional abundance in each of senescent populations from two post-selection HMEC lines (48R and 184) is compared with abundance in isogenic early passage proliferating cells and also with abundance in early passage quiescent cells. A strain or line experiment design type assays differences between multiple strains, cultivars, serovars, isolates, lines from organisms of a single species. Keywords: strain_or_line_design
