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Evolution of neuronal and endothelial transcriptomes in primates

ABSTRACT: Neurons and endothelial cells were identified by immunohistochemistry in human brains, isolated by laser-capture-microdissection and used to find genes preferentially expressed in the two cell types. Keywords: cell type comparison

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE12293 | GEO | 2010/05/20

SECONDARY ACCESSION(S): PRJNA113585

REPOSITORIES: GEO

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Evolution of neuronal and endothelial transcriptomes in primates

Project description:Neurons and endothelial cells were identified by immunohistochemistry in human brains, isolated by laser-capture-microdissection and used to find genes preferentially expressed in the two cell types. Keywords: cell type comparison Laser capture microdissection was used to isolate approximately 1000 neurons and endothelial cells from 6 (for neurons) and 7 (for endothelial cells) human post mortem brain samples. RNA was isolated and amplified (3 linear amplifications) and genome wide expression was measured. This allowed the identification of genes that are differentially expressed between neurons and endothelial cells in the human brain.

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Evolution of neuronal and endothelial transcriptomes in primates.

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Evolution of neuronal and endothelial transcriptomes in primates

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