Effect of HFD exposure on hepatic transcriptome on Wild Type mice (WT), hepatocyte-specific PPARα knockout mice (LKO) and whole body PPARα knockout mice (KO).
Ontology highlight
ABSTRACT: Adult (12 weeks old) WT, LKO and KO male mice from C57Bl6J were either treated with a control diet (CTRL) or an High Fat Diet (HFD) during 12 weeks prior to liver gene expression analysis
Project description:To investigate gene expression changes induced by LPIAT1 depletion, Lpiat1F/F and LKO mice were fed high fat diet (HFD) or chow for 18 weeks from 6 weels old and microarray gene profiling were performed. Categorization of the differentially expressed genes between HFD-fed Lpiat1f/f and LKO mice, in relation to their functions in biological pathways, revealed an enrichment of genes involved in inflammation and fibrosis
Project description:Effect of HFD exposure on hepatic transcriptome on Wild Type mice (WT), hepatocyte-specific PPARα knockout mice (LKO) and whole body PPARα knockout mice (KO).
Project description:RNA Sequencing Analysis of Control and Blnc1 liver specific knockout mice (LKO) Liver Transcriptomes after 24 weeks NASH diet feeding
Project description:LXR is a transcription factor. Two isoforms exist in mice (alpha and beta). They are both expressed in the liver. We examined the role of LXR by obtaining gene expression profiles from the livers of wild-type and LXR-/- mice from the same genetic background. Liver gene expression was measured from male mice (5 mice/group) of genotypes wild-type and LXR-/- on a mixed Sv129/C57Bl6J background (Repa et al. , 2000, Science, 289(5484):1524-9; Cummins et al., 2006, J Clin Invest,116(7):1902-12) fed for 7 weeks a normal diet (ND) or a Western diet (WD).
Project description:Aortic arch profiling of Ldlr-/- huCETP transgenic mice fed a low fat, cholesterol-containing western diet (LFWD, 9% fat, 0.15% cholesterol) starting at 8 weeks of age for 4, 8, 12, and 16 weeks.
Project description:Our quantitative RT-PCR analysis suggested a significant inverse relationship between PPARα activation and let-7 expression in mouse liver. Phenotypic analysis of hepatocyte-specific let-7b/c2 knockout (let7b/c2 LKO) mice showed significnat less weight gain after high-fat diet (HFD) feeding. To discover potential clues for the physiological role of let-7 miRNA in liver, RNA-seq analysis was performed in let-7b/c2 LKO mouse mice fed a HFD. Our data revealed that Let-7 miRNA disruption in hepatocytes resulted in suppression of PPARα signaling.
Project description:C57BL/6 male mice were fed a standardized NCD or HFD after weaning (4 weeks of age) over a course of 12 weeks. Normal chow diet (NCD) fed animals served as Controls
Project description:Purpose: Aim of the study is to identify changes in hepatic gene expression induced by either a 40kcal% coconut oil rich high fat diet (HFD), a 40kcal% soybean oil plus coconut oil high fat diet (SO-HFD) or a low fat vivarium chow diet (Viv). Methods: Livers from mice that had been fed one of the above mentioned diets for 35 weeks, were used to make cDNA libraries that were then sent for deep sequencing, using the Illumina TruSeq RNA. Result: Many genes involved in metabolism, lipid binding, transport and storage and many Cyp genes are dysregulated in the two high fat diets as compared to Viv HFDs in SO-HFD mice. Comparing the two HFDs shows more metabolism and disease related genes dysregulated in SO-HFD vs HFD. Conclusion: A diet high in soybean oil may be more detrimental to metabolic health than a diet high in saturated fats. cDNA isolated from livers from mice fed HFD, SO-HFD or Viv for 35 weeks, were 50bp pair-ended sequenced in triplicate using Illumina TruSeq RNA Sample Prep v2 Kit.