Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MicroRNA responses of LPS and IL-10 stimulated primary human monocyte-derived macrophages over a short time-course

ABSTRACT: Lipopolysaccharide stimulated primary human monocyte-derived macrophages at 2, 4 and 8hrs, IL-10 at 4hrs, IL-10+LPS at 4hrs

ORGANISM(S): Human gammaherpesvirus 8 Mus musculus JC polyomavirus Rattus norvegicus Betapolyomavirus macacae Homo sapiens Human immunodeficiency virus 1 Murid gammaherpesvirus 4 Human betaherpesvirus 5 Human alphaherpesvirus 1 Betapolyomavirus hominis human gammaherpesvirus 4 Murid betaherpesvirus 1

PROVIDER: GSE125572 | GEO | 2019/01/25

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

IL-10 or IL-6 stimulation of IL-6-/- and IL-10-/- murine bone marrow derived macrophages in the presence of LPS

Project description:IL-10 or IL-6 stimulation of control 129xC57BL/6 murine bone marrow derived macrophages in the presence of LPS. We used microarrays to detail the global programme of gene expression changes in response to IL-6 or IL-10 stimulation in the presence of lipopolysaccharide. BMDMs were isolated from control, IL-6-/-, and IL-10-/- mice on a 129XBL/6 mixed background mice and differentiated in the presence of CSF-1 for 6-7 days. Cells were scraped and plated in 6 well plates at 2x10e6/well. Cells were washed with complete DMEM and rested for 1-2 hr before stimulation with combinations of IL-10 (10 ng/ml), IL-6 (2 ng/ml) or LPS (100 ng/ml) for 45 min or 180 mins. Complete biological replicates were performed. Keywords: time course

2006-08-25 | GSE5589 | GEO

microRNA responses of LPS and IL-10 stimulated primary human monocyte-derived macrophages over a short time-course

Project description:microRNA responses of LPS and IL-10 stimulated primary human monocyte-derived macrophages over a short time-course

| PRJNA516857 | ENA

Transcription profiling of mouse bone marrow macrophages from IL-6-/- and IL-10-/- mutants treated with IL-10 or IL-6 stimulation in the presence of LPS

2008-06-13 | E-GEOD-5589 | biostudies-arrayexpress

Transcription profiling of human monocyte derived LPS-activated activated dendritic cells treated with IL-10

Project description:Gene expression profiels in the human monocyte-derived dendritic cells (DCs) from 4 different donors (A, B, C, and D) were studied. Cells were left untreated (Group 4), activated with LPS alone (Group 1) or activated in the presence cmv IL-10 (Group 2) or human IL-10 (Group 3) for 12 hours before subjected to RNA extraction. Experiment Overall Design: Overall ranscriptional profiles of activated DCs and IL-10-exposed activated DCs were compared with untreaetd immature DCs as baseline control.

2008-06-15 | E-GEOD-7095 | biostudies-arrayexpress

IL-10

Project description:IL-10

| PRJNA1061148 | ENA

RNA-seq of M-CSF differentiated human peripheral monocyte-derived macrophages (MDMs) activated with short exposure (3hours) to LPS, or long exposure (24 hours) to LPS, LPS with IFNγ, IFNγ, IL-4, IL-10, and dexamethasone

Project description:The goal of this experiment was to validate a classification model built on other bulk transcriptomic data that was capable of identifying in vitro stimulated M-CSF differentiated macrophages. Specifically, the classification model was capable of distinguishing: M-LPSearly (2-4 hours) M-LPSlate (18-24 hours) M-LPS+IFNγ M-IFNγ M-IL4 M-IL-10 M-dex

2020-01-06 | E-MTAB-7572 | biostudies-arrayexpress

Global gene expression changes in patient-derived CML cell line K562 after short- and long-term BCR-ABL kinase inhibition.

Project description:K562 cells were treated with the BCR-ABL kinase inhibitor dasatinib over an extended period of time to determine how BCR-ABL inhibition affects BCR-ABL-dependent negative feedback and erythropoietin receptor (EPO-R) signaling. Specifically, what types of changes (upregulation versus downregulation) occur in both the negative and positive regulators of growth-factor receptor signaling. Total RNA was extracted from K562 cells treated with 0.2% DMSO for 24hrs or 100nM dasatinib for 4hrs, 8hrs, and 24hrs.

2013-09-24 | E-GEOD-51083 | biostudies-arrayexpress

Drosophila melanogaster whole embryo 3'-Tag-Seq experiments during embryogenesis

Project description:Sequencing of the most 3' end of mRNAs (3'-Tag-Seq) of 82 wild type strains from the Drosophila Genetic reference Panel (DGRP) during embryogenesis was performed. Three different time points (2-4hrs, 6-8hrs and 10 to 12rs after laying) were assayed. 14 biological replicates were performed from independent embryo collections.

2017-01-10 | E-MTAB-4722 | biostudies-arrayexpress

Dendritic cells activated with LPS IFNg over 48 hours

Project description:Pro-inflammation triggered by microbial lipopolysaccharide (LPS) through Toll-like receptor (TLR) 4 in the presence of interferon (IFN)-g induces cytokine secretion in dendritic cells (DCs) tightly regulated by a defined differentiation program. This DC differentiation is characterized by a dynamic immune activating but also tolerance inducing phenotype associated with irreversible down-modulation of cytokines. CD40L on activated T cells further modifies DC differentiation. Using DNA micro arrays we showed down-regulated mRNA levels of TLR signaling molecules while CD40/CD40L signaling molecules were up-regulated at a time when LPS/IFN-g activated DCs have ceased cytokine expression. Accordingly we demonstrated that CD40/CD40L but not TLR4 or TLR3 signaling mediated by LPS or poly (cytidylic-inosinic) acid (poly I:C) and dsRNA re-established the capacity to secret interleukin (IL)-12 in LPS/IFN-g activated DCs, which have exhausted their potential for cytokine secretion. This resulting TH1 polarizing DC phenotype – which lacked accompanying secretion of the crucial immune suppressive IL-10 - enhanced activation of cytotoxic T lymphocytes (CTLs). We therefore conclude that immune modulation is restricted to a secondary T-cell mediated stimulus at an exhausted DC state which prevents an immune tolerant DC phenotype. These findings impacts on the rational design of TLR activated DC-based cancer vaccines for the induction of anti-tumoral CTL responses. Keywords: time course

2009-04-22 | GSE11327 | GEO

5' CAGE at three embryonic times points in 82 Drosophila lines

Project description:Deep sequencing of Transcriptional Start Sites (TSS) using 5' CAGE from 82 wild strains from the Drosophila Genetic reference Panel (DGRP) during embryogenesis. Three different developmental stages were assayed: 2-4hrs (stages 5-8), 6-8hrs (stages 10-11) and 10-12hrs (stages 13-14) after laying. 20 line/stage combinations were performed in biological replicates (independent embryo collections), while 5 samples were prepared as technical replicates (independent library preparation from the same RNA).

2017-01-09 | E-MTAB-4787 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data