Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Characterization of human erythropoiesis using ex vivo differentiation of CD34 HSC

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE125926 | GEO | 2022/06/23

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Characterization of human erythropoiesis using ex vivo differentiation of CD34 HSC (ATAC-seq)

Project description:Genome Wides Association Studies (GWAS) have identified tens of thousands of associations between human genetic variation and common disease. Despite the abundance of GWAS associations, functional identification and characterization of causative variants and effector genes remains a challenging prospect. Human erythropoiesis provides a highly tractable model system for the development of tools for GWAS analysis. We have developed a three-phase protocol for differentiation of peripheral CD34+ Haematopoietic Stem Cells (HSC) into mature enucleating erythrocytes. This protocol has been characterized for it’s the changes observed morphologically, immunologically (by FACS) and epigenetically (by ATAC-seq, ChIP-seq, and RNA-seq). We have used this protocol to study the effects of common GWAS variants affecting red blood cell traits, and more severe mutations causing Type-1 Congenital Dyserythropoietic Anemia (CDA-I).

2021-05-28 | GSE125922 | GEO

Characterization of human erythropoiesis using ex vivo differentiation of CD34 HSC (RNA-seq)

2022-06-22 | GSE125924 | GEO

Characterization of human erythropoiesis using ex vivo differentiation of CD34 HSC (ChIP-seq)

2023-01-21 | GSE125923 | GEO

Characterization of human erythropoiesis using ex vivo differentiation of CD34 HSC (NG Capture-C)

2023-01-21 | GSE125925 | GEO

Characterization of human erythropoiesis using ex vivo differentiation of CD34 HSC

Project description:Characterization of human erythropoiesis using ex vivo differentiation of CD34 HSC

| PRJNA517958 | ENA

Characterization of human erythropoiesis using ex vivo differentiation of CD34 HSC (RNA-seq)

Project description:Characterization of human erythropoiesis using ex vivo differentiation of CD34 HSC (RNA-seq)

| PRJNA517960 | ENA

Characterization of human erythropoiesis using ex vivo differentiation of CD34 HSC (ATAC-seq)

Project description:Characterization of human erythropoiesis using ex vivo differentiation of CD34 HSC (ATAC-seq)

| PRJNA517962 | ENA

Characterization of erythroid cells generated through ex vivo differentiation

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-07-08 | GSE137983 | GEO

DNA methylation and transcriptome dynamics of Flt3L induced ex vivo BMDC differentiation

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-11-06 | GSE107028 | GEO

Enabling Large-Scale Ex Vivo Production of Megakaryocytes from CD34<sup>+</sup> Cells Using Gas-Permeable Surfaces.

Project description:Patients suffering from acute or sustained thrombocytopenia require platelet transfusions, which are entirely donor-based and limited by challenges related to storage and fluctuating supply. Developing cell-culture technologies will enable ex vivo and donor-independent platelet production. However, critical advancements are needed to improve scalability and increase megakaryocyte (Mk) culture productivity. To address these needs, we evaluated Mk production from mobilized peripheral blood CD34+ cells cultured on a commercially available gas-permeable silicone rubber membrane, which provides efficient gas exchange, and investigated the use of fed-batch media dilution schemes. Starting with a cell-surface density of 40 × 103 CD34+ cells per cm2 (G40D), culturing cells on the membrane for the first 5 days and employing media dilutions yielded 39 ± 19 CD41+ CD42b+ Mks per input CD34+ cell by day 11-a 2.2-fold increase compared with using standard culture surfaces and full media exchanges. By day 7, G40D conditions generated 1.5-fold more CD34+ cells and nearly doubled the numbers of Mk progenitors. The increased number of Mk progenitors coupled with media dilutions, potentially due to the retention of interleukin (IL)-3, increased Mk production in G40D. Compared with controls, G40D had higher viability, yielded threefold more Mks per milliliter of media used and exhibited lower mean ploidy, but had higher numbers of high-ploidy Mks. Finally, G40D-Mks produced proplatelets and platelet-like-particles that activate and aggregate upon stimulation. These results highlight distinct improvements in Mk cell-culture and demonstrate how new technologies and techniques are needed to enable clinically relevant production of Mks for platelet generation and cell-based therapies.

| S-EPMC6591548 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data