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A positive, growth-based PAM screen reveals non-canonical motifs recognized by the S. pyogenes Cas9

ABSTRACT: We report non-canonical PAM sequences for SpyCas9 that were revealed from a novel growth-based PAM screen in E. coli.

ORGANISM(S): Escherichia coli Homo sapiens

PROVIDER: GSE125959 | GEO | 2020/06/25

REPOSITORIES: GEO

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A positive, growth-based PAM screen identifies noncanonical motifs recognized by the <i>S. pyogenes</i> Cas9.

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