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Deep sequencing data for PAM-SCANR tested CRISPR-Cas systems

ABSTRACT: In this work, we analyzed recognized PAM sequences from four CRISPR-Cas systems: E. coli I-E, B. halodurans I-C, S. thermophilus CR1 II-A, and F. novicida V. Cells containing functional PAMs were sorted using FACS and subsequently sequenced.

ORGANISM(S): Streptococcus thermophilus Escherichia coli Francisella tularensis subsp. novicida Halalkalibacterium halodurans

PROVIDER: GSE75718 | GEO | 2016/02/17

SECONDARY ACCESSION(S): PRJNA305147

REPOSITORIES: GEO

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Deep sequencing data for PAM-SCANR tested CRISPR-Cas systems

Project description:In this work, we analyzed recognized PAM sequences from four CRISPR-Cas systems: E. coli I-E, B. halodurans I-C, S. thermophilus CR1 II-A, and F. novicida V. Cells containing functional PAMs were sorted using FACS and subsequently sequenced. Ranking of recognized PAMs from a positive screen

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Deep sequencing data for PAM-SCANR tested CRISPR-Cas systems

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