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Randomized PAM depeletion of NmeCas9

ABSTRACT: We report the PAMs of NmeCas9 using a cell-free TXTL-based cleavage assay. By adding randomized PAM library and NmeCas9-gRNA in vitro, functional PAM sequences were cleaved, while non-functional PAMs remained. By amplifying the non-cleaved DNA, we use next-generation sequencing to analyze the depletion of functional PAMs of NmeCas9.

ORGANISM(S): synthetic construct

PROVIDER: GSE108357 | GEO | 2017/12/21

SECONDARY ACCESSION(S): PRJNA423256

REPOSITORIES: GEO

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