Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Analysis of ARID3A and ARID3B regulated genes by RNA-seq

ABSTRACT: In ovarian cancer cells ARID3A, ARID3B, or both genes were expressed exogenously with lentivirus. Cells were sorted based on expression level using fluoescent tags. RNA was isolated and RNA-sequencing was conducted to identify ARID3 regulated genes.

ORGANISM(S): Homo sapiens

PROVIDER: GSE129082 | GEO | 2020/02/05

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Analysis of ARID3A and ARID3B regulated genes by RNA-seq

Project description:Analysis of ARID3A and ARID3B regulated genes by RNA-seq

| PRJNA529973 | ENA

Genome-wide characterization of ARID3B binding sites and KDM4C in ARID3B-knockout OECM1 cells

Project description:In this study we assayed for genome-wide localization of ARID3B and KDM4C enrichment in control and ARID3B-knockout OECM1 cells. The expression of the embryonic stem cell (ESC) signature in cancer cells indicates the coordinated regulation of the stemness genes in cancer stem cells, which are responsible for cancer initiation and dissemination. let-7 family microRNAs are crucial regulators for stem cell differentiation. In cancer cells, let-7 suppresses cancer stemness through targeting different oncogenes such as c-Myc, RAS, and HMGA2. However, most let-7 target genes are oncogenes rather than stemness factors, and the mechanism of let-7-repressed stemness is unclear. Here we demonstrate that let-7 supresses the formation of AT-rich interacting domain 3B (ARID3B) complex through targeting the expression of ARID3B, the interacting partner ARID3A, and importin 9. ARID3B complex recruits histone demethylase 4C (KDM4C) to the regulatory region of stemness genes for reducing histone 3 lysine 9 trimethylation, resulting in an open configuration of the chromatin of stemness genes. In cancer tissues, ARID3B expression correlates with the nuclear ARID3A expression and a worse prognosis. This result highlights the role of let-7 in regulating stemness through histone modifications.

2015-12-01 | GSE66601 | GEO

Analysis of ARID3A regulated genes in K562 cells

Project description:To test the function and regulated genes of ARID3A gene, we conducted lentivirus-mediated short hairpin RNA (shRNA) against ARID3A in K562 cell line.

2023-06-01 | E-MTAB-12603 | biostudies-arrayexpress

The mRNA expression of FaDu-Ctrl, FaDu-ARID3B cells

Project description:Microarray analyses for the identification of differences in gene expression patterns have increased our understanding of the molecular mechanism of ARID3B in HNSCC.We used gene expression analysis data from FaDu-ARID3B and FaDu-pCDH to identify differentially expressed probes. The expression of the embryonic stem cell (ESC) signature in cancer cells indicates the coordinated regulation of the stemness genes in cancer stem cells, which are responsible for cancer initiation and dissemination. let-7 family microRNAs are crucial regulators for stem cell differentiation. In cancer cells, let-7 suppresses cancer stemness through targeting different oncogenes such as c-Myc, RAS, and HMGA2. However, most let-7 target genes are oncogenes rather than stemness factors, and the mechanism of let-7-repressed stemness is unclear. Here we demonstrate that let-7 supresses the formation of AT-rich interacting domain 3B (ARID3B) complex through targeting the expression of ARID3B, the interacting partner ARID3A, and importin 9. ARID3B complex recruits histone demethylase 4C (KDM4C) to the regulatory region of stemness genes for reducing histone 3 lysine 9 trimethylation, resulting in an open configuration of the chromatin of stemness genes. In cancer tissues, ARID3B expression correlates with the nuclear ARID3A expression and a worse prognosis. This result highlights the role of let-7 in regulating stemness through histone modifications.

2015-12-01 | GSE66602 | GEO

CMK LC-MS/MS after CoIP of ARID3A

Project description:In order to determine proteins interacting with ARID3A in the ML-DS cell line CMK, we performed CoIP of endogenous ARID3A followed by LC-MS/MS

2023-03-11 | PXD025027 | Pride

Gene expression profile of ARID3B knockout in HCT15 cells

Project description:Microarray analyses for identification of the differences in gene expression patterns have increased our understanding of the molecular mechanism of ARID3B in colon caner cellls.We used gene expression analysis data from HCT15-ARID3B-Cas9 and HCT15-Ctrl to identify differentially expressed probes.

2021-12-27 | GSE92838 | GEO

Hepatic ARID3A facilitates liver cancer malignancy by cooperating with CEP131 to regulate an embryonic stem cell-like gene signature

Project description:Huh 7 cells were infected with lentivirus or transfected with siRNA using Lipofectamine RNAiMAX to overexpress or knockdown indicated genes. Chromatin immunoprecipitation followed by sequencing (ChIP-seq) was performed using indicated antibodies to study the H3K9me2 status after certain treatment in Huh7 cells. Cleavage Under Targets and Tagmentation (CUT&Tag) was performed to explore the chromatin binding sites of ARID3A. Whole-transcriptome sequencing (RNA-Seq) was performed to identify the downstream target genes regulated by ARID3A or KDM3A in Huh 7 cells.

2022-08-10 | GSE193726 | GEO

Gene expression in E9.5 trunks comparing wt with Arid3b-null embryos

Project description:We have analysed and compared mRNA expression between wt embryos and embryos deficient for Arid3b in E9.5 trunks, with the aim of identifying differentially expressed genes that could give us a clue to the functions of Arid3b during development.

2014-10-07 | GSE62071 | GEO

Gene expression in E9.5 heads comparing wt with Arid3b-null embryos

Project description:We have analysed and compared mRNA expression between wt embryos and embryos deficient for Arid3b in E9.5 heads, with the aim of identifying differentially expressed genes that could give us a clue to the functions of Arid3b during development.

2014-10-07 | GSE62070 | GEO

Gene expression in E9.5 hearts comparing wt with Arid3b-null embryos

Project description:We have analysed and compared mRNA expression between wt embryos and embryos deficient for Arid3b in E9.5 hearts, with the aim of identifying differentially expressed genes that could give us a clue to the functions of Arid3b during development.

2014-10-07 | GSE62069 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data