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Genome-wide interaction map of YtrA transcription factor in S. Acidocaldarius

ABSTRACT: We report the characterization of GntR-like regulator in the Archaeon Sulfolobus acidocaldarius by looking at the genome wide interaction map of the protein in vivo

ORGANISM(S): Sulfolobus acidocaldarius

PROVIDER: GSE129461 | GEO | 2019/09/26

REPOSITORIES: GEO

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Next generation sequencing to identify differentially expressed genes in the YtrASa overexpression strain

Project description:We report the regulon of a GntR-like regulator from the archaeon Sulfolobus acidocaldarius by looking at differentially expressed genes comparing a YtrASa overexpression strain with its isogenic wild ype

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GntR-like regulator in the Archaeon Sulfolobus acidocaldarius

Project description:GntR-like regulator in the Archaeon Sulfolobus acidocaldarius

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Identification of the Sulfolobus acidocaldarius small RNome and L7Ae-RNA interactome

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Project description:Sulfolobus acidocaldarius is an obligate aerobe that grows in hot and acidic environments. S. acidocaldarius have been reported to grow on a variety of organic compounds as carbon and energy sources. However, little is known about systemic elucidation of carbon utilization for biomass formation and energy metabolism in S. acidocaldarius. In this analysis, the effect of glucose on genome-wide transcriptional profiling in S. acidocaldarius DSM 639 was investigated by RNA-Seq technology.

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Sulfolobus acidocaldarius phosphoproteome

Project description:Shotgun phosphoproteome of Sulfolobus acidocaldarius using PAciFIC technique. Briefly, proteins were denatured, alkylated, trypsine digestion, SCX separation Fractionated peptides were analysed on Bruker HCTultra. Data processing and bioinformatics: data from mass spectrometry were then extracted to mgf format using Bruker Data Analysis V4.0 with a MRM script, these were then were searched against the Sulfolobus acidocaldarius database (containing 2223 proteins) downloaded from NCBI in March 2010 using Phenyx V 2.6 (Genebio, Geneva). The searches were performed using parameters as follows: carbamidomethylation of cysteine (fixed modification), oxidation of methionine (variation), and phosphorylation of serine, tyrosine, threonine (variation), trypsin with 2 missed cleavages. Furthermore, other parameters such as parent, MS/MS, tolerances were set at 2.0 and 0.8 Da, respectively, whilst minimum peptide length, z-score, p-value and AC score were set at 5, 5.5, 10-5, and 5.5 respectively.

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The genome-scale DNA-binding of FadR transcription factor in Sulfolobus acidocaldarius

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Project description:Sulfolobus acidocaldarius Raw sequence reads

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