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Next generation sequencing to identify differentially expressed genes in the YtrASa overexpression strain

ABSTRACT: We report the regulon of a GntR-like regulator from the archaeon Sulfolobus acidocaldarius by looking at differentially expressed genes comparing a YtrASa overexpression strain with its isogenic wild ype

ORGANISM(S): Sulfolobus acidocaldarius

PROVIDER: GSE129463 | GEO | 2019/09/26

REPOSITORIES: GEO

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Genome-wide interaction map of YtrA transcription factor in S. Acidocaldarius

Project description:We report the characterization of GntR-like regulator in the Archaeon Sulfolobus acidocaldarius by looking at the genome wide interaction map of the protein in vivo

2019-09-26 | GSE129461 | GEO

GntR-like regulator in the Archaeon Sulfolobus acidocaldarius

Project description:GntR-like regulator in the Archaeon Sulfolobus acidocaldarius

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Transcriptome of S. acidocaldarius MW001 strain grown as biofilm or planktonic cells and transcriptomic profile of an RNaseR-treated S. acidocaldarius MW001 small RNA sample

Project description:Total RNAs were isolated from the thermophilic archaeon Sulfolobus acidocaldarius MW001 and subjected to Illumina sequencing. A differential gene expression analysis was performed to identify ncRNA in biofilm- associated cells and planktonically grown cells. Additionally, the small RNA subpopulation of the transcriptome was subjected to RNaseR digestion prior to Illumina sequencing. An abundant double-stranded RNA was identified.

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The genome-scale DNA-binding profile of BarR

Project description:The Leucine-responsive Regulatory Protein (Lrp) family is a widespread family of regulatory transcription factors in prokaryotes. BarR is an Lrp-like transcription factor in the model archaeon Sulfolobus acidocaldarius that activates the expression of a -alanine aminotransferase gene, which is involved in -alanine degradation. In contrast to classical Lrp-like transcription factors, BarR is not responsive to any of the -amino acids but interacts specifically with -alanine. Besides the juxtaposed -alanine aminotransferase gene, other regulatory targets of BarR have not yet been identified although -alanine is the precursor of coenzyme A and thus an important central metabolite. The aim of this study is to extend the knowledge of the DNA-binding characteristics of BarR and of its corresponding regulon from a local to a genome-wide perspective.

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Transcription profiling of hyperthermophilic crenarchaea Sulfolobus solfataricus and Sulfolobus acidocaldarius to UV irradiation

Project description:Analysis of transcriptional response to UV irradiation in two related crenarchaea, Sulfolobus solfataricus and Sulfolobus acidocaldarius.

2007-11-21 | E-MEXP-1252 | biostudies-arrayexpress

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Project description:Small RNAs and L7Ae co-immunoprecipitated RNA were isolated from the thermophilic archaeon Sulfolobus acidocaldarius and subjected to Illumina sequencing. The sRNome revealed a high abundance of C/D box sRNAs and CRISPR RNAs. The L7Ae-RNA interactome showed enrichment of all known interactors of the L7Ae protein, i.e. C/D box sRNAs, H/ACA box sRNAs, RNaseP, rRNA. A high abundance of reads for the SRP RNA was found, suggesting L7Ae´s interaction with this universal RNA. Many mRNA reads were enriched, several of them comprise binding sites for the L7Ae protein.

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Transcriptome analysis of Sulfolobus acidocaldarius DSM 639 under sugar-free and sugar-rich conditions

Project description:Sulfolobus acidocaldarius is an obligate aerobe that grows in hot and acidic environments. S. acidocaldarius have been reported to grow on a variety of organic compounds as carbon and energy sources. However, little is known about systemic elucidation of carbon utilization for biomass formation and energy metabolism in S. acidocaldarius. In this analysis, the effect of glucose on genome-wide transcriptional profiling in S. acidocaldarius DSM 639 was investigated by RNA-Seq technology.

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MPLEx_Sulfolobus_acidocaldarius

Project description:Datasets of Sulfolobus acidocaldarius samples used to test the MPLEx method.

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Transcriptional Response of 0.1%YE-grown M. sedula cells to ferrous sulfate

Project description:(from abstract): Iron oxidation is a desirable trait of biomining microorganisms, although the mechanism is not well-understood in extreme thermoacidophiles. The complete genome sequence of the extremely thermoacidophilic archaeon Metallosphaera sedula DSM 5348 (2.2 Mb, ~2300 ORFs) provides insights into biologically catalyzed metal sulfide oxidation. Comparative genomics was used to identify pathways and proteins (in)directly involved with bioleaching. As expected, the M. sedula genome encodes genes related to autotrophic carbon fixation, metal tolerance, and adhesion. Also, terminal oxidase cluster organization indicates the presence of hybrid quinol-cytochrome oxidase complexes. Comparisons with the mesophilic biomining bacterium Acidithiobacillus ferrooxidans ATCC 23270 indicate that the M. sedula genome encodes at least one putative rusticyanin, involved in iron oxidation. The fox gene cluster, involved in iron oxidation in the thermoacidophilic archaeon Sulfolobus metallicus, could also be identified. These iron-oxidizing components are missing from genomes of non-leaching Sulfolobales like Sulfolobus solfataricus P2 and Sulfolobus acidocaldarius DSM 639. Whole genome transcriptional response analysis showed that 88 ORFs were up-regulated 2-fold or more in M. sedula upon addition of ferrous sulfate to yeast extract-based medium; these included components of terminal oxidase clusters predicted to be involved with iron oxidation, as well as genes predicted to be involved with sulfur metabolism. Many hypothetical proteins were also differentially transcribed, indicating that aspects of the iron and sulfur metabolism of M. sedula remain to be identified and characterized. Keywords: substrate response

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Whole-cell proteome of Sulfolobus acidocaldarius strain 98-3

Project description:Identification of the proteins from tryptic digests of Sulfolobus acidocaldarius strain 98-3 whole-cell proteome followed by nanoLC/OrbitrapFusion MS analysis.

2024-07-16 | PXD051007 | JPOST Repository

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