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Transcriptional Idenetity of Itch-activated Central Amygdala Neurons

ABSTRACT: We performed RNA-seq to identify transcriptional profiles of itch-activated CeA neurons. To do this, we crossed FosTRAP mice to the Cre-dependent tdTomato flox-stop reporter line as described above. We then injected chloroquine into the nape of the neck, paired with injection of 4-OHT to FosTRAP expression of tdTomato in itch-activated neurons. The CeA was harvested, the cells dissociated and sorted by FACS, and tdTomato+ cells were compared to TdTomato- cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE130268 | GEO | 2020/04/23

REPOSITORIES: GEO

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Transcriptional Idenetity of Itch-activated Central Amygdala Neurons

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