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Effect of AICAR and SAICAR accumulation on global transcription (first part)

ABSTRACT: We investigated the effects of AICAR and SAICAR accumulation on transcriptional regulation of purine biosynthesis and other metabolic pathways. In this work, global transcription analyses were combined to measurements of intracellular nucleotides pools by HPLC. Keywords: Comparative genomic transcription analysis on total RNA from cells accumulating or not AICAR and SAICAR nucleotide derivatives.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE13182 | GEO | 2009/05/15

SECONDARY ACCESSION(S): PRJNA114097

REPOSITORIES: GEO

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Effect of AICAR and SAICAR accumulation on global transcription (second part)

Project description:We investigated the effects of AICAR and SAICAR accumulation on transcriptional regulation of purine biosynthesis and other metabolic pathways. These data were compared to those obtained in the ade16ade17 vs WT data set to determine the transcriptional effects associated to AICAR/SAICAR accumulation and those due to ade16 and ade17 deletions. In this work, global transcription analyses were combined to measurements of intracellular nucleotides pools by HPLC Keywords: Comparative genomic transcription analysis on total RNA from cells accumulating or lacking AICAR and SAICAR nucleotide derivatives.

2009-05-15 | GSE13183 | GEO

Role of the Bas1p transcription factor in the transcriptional response associated to AICAR and SAICAR accumulation

Project description:We investigated the role of Bas1p in the transcriptional response due to AICAR and SAICAR accumulation. In this work, global transcription analyses were combined to measurements of intracellular nucleotides pools by HPLC. Keywords: Comparative genomic transcription analysis on total RNA from cells accumulating AICAR and SAICAR in a bas1 deletion context.

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Role of the Pho4p transcription factor in the transcriptional response associated to AICAR and SAICAR accumulation

Project description:We investigated the role of Pho4p in the transcriptional response associated to AICAR and SAICAR accumulation. In this work, global transcription analyses were combined to measurements of intracellular nucleotides pools by HPLC. Keywords: Comparative genomic transcription analysis on total RNA from cells accumulating AICAR and SAICAR in a pho4 deletion context.

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Role of the Pho2p transcription factor in the transcriptional response associated to AICAR and SAICAR accumulation

Project description:We investigated the role of Pho2p in the transcriptional response associated to AICAR and SAICAR accumulation. In this work, global transcription analyses were combined to measurements of intracellular nucleotides pools by HPLC. Keywords: Comparative genomic transcription analysis on total RNA from cells accumulating AICAR and SAICAR in a pho2 deletion context.

2009-05-15 | GSE13185 | GEO

Role of the Snf1p protein kinase in the transcriptional response associated to AICAR and SAICAR accumulation

Project description:We investigated the role of Snf1p in the transcriptional response associated to AICAR and SAICAR accumulation. In this work, global transcription analyses were combined to measurements of intracellular nucleotides pools by HPLC. Keywords: Comparative genomic transcription analysis on total RNA from cells accumulating or not AICAR and SAICAR nucleotide derivatives in a snf1 deletion context.

2009-05-15 | GSE13188 | GEO

Effect of SAICAR accumulation on global transcription

Project description:We investigated the effects of SAICAR accumulation on transcriptional regulation of purine biosynthesis and other metabolic pathways. These data were compared to those obtained in the ade16ade17 vs WT comparison to determine the effects associated to accumulation of SAICAR alone. In this work, global transcription analyses were combined to measurements of intracellular nucleotides pools by HPLC. Keywords: Comparative genomic transcription analysis on total RNA from cells accumulating or not SAICAR.

2009-05-15 | GSE13184 | GEO

Effect of AICAr and SAICAr accumulation on global transcription in the ade3 mutant

Project description:We investigated the effects of AICAr and SAICAr accumulation on the global transcriptome of yeast, comparing the ade3 mutant to the WT strain. The ade3 mutant strain (Y4143) was compared to the WT strain (Y2946). For each comparison, we made 2 technical replicates, swapping dyes.

2011-08-31 | E-GEOD-29021 | biostudies-arrayexpress

Effect of AICAr and SAICAr accumulation on global transcription in the ade3 mutant

Project description:We investigated the effects of AICAr and SAICAr accumulation on the global transcriptome of yeast, comparing the ade3 mutant to the WT strain.

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Effect of AICAR monophosphate and AICAr riboside accumulation on global transcription

Project description:AICAR monophosphate (5-Aminoimidazole-4-carboxamide ribonucleotide) is a metabolic intermediate of the de novo purine synthesis pathway presenting highly promising metabolic and antiproliferative properties. Yeast mutants accumulating AICAR are auxotroph for histidine. A screening for suppressors of this phenotype identified recessive and dominant mutants that result in lowering intracellular AICAR concentration. The recessive mutants affect adenosine kinase which is shown here to catalyze the phosphorylation of AICAR riboside in yeast. The dominant mutants strongly enhance the capacity of the alkaline phosphatase Pho13p to dephosphorylate succinyl-AICAR monophosphate into the non-toxic riboside form. By combining these mutants to transcriptomic and metabolomic analyses, we establish that in yeast both toxic and physiological responses to AICAR are linked to the concentration of the monophosphate form, while the nucleoside moiety has no effect even at high concentration. Finally, we establish that (S)AICAR concentration varies under physiological conditions, thus modulating transcriptional regulation of purine pathway genes. This SuperSeries is composed of the SubSeries listed below.

2011-07-11 | GSE29324 | GEO

Transcriptome of primary acute myeloid leukemia (AML) cells treated with AICAr

Project description:Ribonucleoside AICAr, a precursor in purine synthesis and a known activator of AMPK, was shown to induce monocytic differentiation in AML cell lines by inducing pyrimidine depletion followed by activation of ATR/Chk1 pathway and cell cycle arrest. Here we report transcriptional changes induced by AICAr in the primary AML sample (FAB-M2, FLT3 negative, NPM1 negative) in which AICAr induced differentiation and accumulation of macrophage cells in vitro. RNA-seq transcriptome analysis of primary AML cells isolated from the bone marrow of a patient with de novo FAB-M2 AML. Mononuclear cells were isolated from the bone marrow sample by density gradient separation and grown in RPMI supplemented with 10% FBS, 2 mM L-glutamine, 50 U/ml penicillin, 50 μg/ml streptomycin and 50 ng/ml IL-3, IL-6, SCF and FLT3L. Cells were treated with AICAr (0.4 mM) for 24 h. Experiment was performed in biological triplicates and total RNA was isolated for transcriptome analysis using Illumina high throughput sequencing.

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