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Role of the Pho2p transcription factor in the transcriptional response associated to AICAR and SAICAR accumulation

ABSTRACT: We investigated the role of Pho2p in the transcriptional response associated to AICAR and SAICAR accumulation. In this work, global transcription analyses were combined to measurements of intracellular nucleotides pools by HPLC. Keywords: Comparative genomic transcription analysis on total RNA from cells accumulating AICAR and SAICAR in a pho2 deletion context.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE13185 | GEO | 2009/05/15

SECONDARY ACCESSION(S): PRJNA114103

REPOSITORIES: GEO

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Effect of AICAR and SAICAR accumulation on global transcription (first part)

Project description:We investigated the effects of AICAR and SAICAR accumulation on transcriptional regulation of purine biosynthesis and other metabolic pathways. In this work, global transcription analyses were combined to measurements of intracellular nucleotides pools by HPLC. Keywords: Comparative genomic transcription analysis on total RNA from cells accumulating or not AICAR and SAICAR nucleotide derivatives.

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Role of the Bas1p transcription factor in the transcriptional response associated to AICAR and SAICAR accumulation

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Role of the Pho4p transcription factor in the transcriptional response associated to AICAR and SAICAR accumulation

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Effect of AICAR and SAICAR accumulation on global transcription (second part)

Project description:We investigated the effects of AICAR and SAICAR accumulation on transcriptional regulation of purine biosynthesis and other metabolic pathways. These data were compared to those obtained in the ade16ade17 vs WT data set to determine the transcriptional effects associated to AICAR/SAICAR accumulation and those due to ade16 and ade17 deletions. In this work, global transcription analyses were combined to measurements of intracellular nucleotides pools by HPLC Keywords: Comparative genomic transcription analysis on total RNA from cells accumulating or lacking AICAR and SAICAR nucleotide derivatives.

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Project description:We investigated the role of Snf1p in the transcriptional response associated to AICAR and SAICAR accumulation. In this work, global transcription analyses were combined to measurements of intracellular nucleotides pools by HPLC. Keywords: Comparative genomic transcription analysis on total RNA from cells accumulating or not AICAR and SAICAR nucleotide derivatives in a snf1 deletion context.

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Effect of SAICAR accumulation on global transcription

Project description:We investigated the effects of SAICAR accumulation on transcriptional regulation of purine biosynthesis and other metabolic pathways. These data were compared to those obtained in the ade16ade17 vs WT comparison to determine the effects associated to accumulation of SAICAR alone. In this work, global transcription analyses were combined to measurements of intracellular nucleotides pools by HPLC. Keywords: Comparative genomic transcription analysis on total RNA from cells accumulating or not SAICAR.

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Project description:We investigated the effects of AICAr and SAICAr accumulation on the global transcriptome of yeast, comparing the ade3 mutant to the WT strain. The ade3 mutant strain (Y4143) was compared to the WT strain (Y2946). For each comparison, we made 2 technical replicates, swapping dyes.

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Effect of AICAr and SAICAr accumulation on global transcription in the ade3 mutant

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Effect of AICAR monophosphate and AICAr riboside accumulation on global transcription

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Project description:This SuperSeries is composed of the SubSeries listed below.

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