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Endometriosis: normal and ectopic endometrium

ABSTRACT: To explore the difference between ectopic and normal endometrium and find out the characteristics and critical changes in ectopic endometrium, we analyzed transcripts of stromal cells from two groups of patients. The ectopic endometrium and normal endometrium had similar tissue structures and compositions, unlike normal ovaries. The similarity of gene expression profile between the two groups is as high as 97.2%, only 2.8% is significantly different. The differentially expressed genes in the ectopic endometrium are mainly enriched in the adhesion process and the adhesion pathway, which has the adhesion characteristics. Among these differentially expressed genes, CNTN1 is the most variable and participates in multiple pathogenic pathways, which is considered as the core gene to enhance stromal cell adhesion. The up-regulated expression of CNTN1 in all stages of ectopic endometrium is confirmed.

ORGANISM(S): Homo sapiens

PROVIDER: GSE132464 | GEO | 2022/05/01

REPOSITORIES: GEO

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Project description:The pathophysiology of endometriotic lesion development remains unclear but involves a complex interaction between ectopic endometrium and host peritoneal tissues. We hypothesised that disruption of this interaction was likely to suppress endometriotic lesion formation. We hoped to delineate the molecular and cellular dialogue between ectopic human endometrium and peritoneal tissues in nude mice, as a first step towards testing this hypothesis. Human endometrium was xenografted into nude mice and the resulting lesions were analysed using microarrays. A novel technique was developed that unambiguously determined whether RNA transcripts identified by the microarray analyses originated from human cells (endometrium) or mouse cells (stroma). Four key pathways (ubiquitin/proteosome, inflammation, tissue remodelling/repair and ras-mediated oncogenesis) were revealed, that demonstrated communication between host stromal cells and ectopic endometrium. Morphometric analysis of nude mouse lesions confirmed that necrosis, inflammation, healing and repair and cell proliferation occurred during xenograft development. These processes were entirely consistent with the molecular networks revealed by the microarray data. The transcripts detected in the xenografts overlapped with transcripts differentially expressed in a comparison between paired eutopic and ectopic endometrium from human endometriotic patients. For the first time components of the interaction between ectopic endometrium and peritoneal stromal tissues have been revealed in ectopic endometrial lesions. Targeted disruption of this dialogue is likely to disrupt endometriotic tissue formation and may prove to be an effective therapeutic strategy for endometriosis. Experiment Overall Design: Xenografts of human endometrium in nude mice hybridised to both human and mouse specific genechips. 2 biological replicates, at 3 time points. Control human endometrium and mouse pertioneum were hybridised to both human and mouse genechips

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Endometriosis: normal and ectopic endometrium

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